Correlation Between Fat Mass And Obesity-Associated Gene FTO Rs9939609 Polymorphism And Glucose Abnormalities Among The Long-Term Aerobic Exercise Elderly

ObjectiveThis study selected 323 long-term aerobic exercise elderly in Shanghai as the research object, to explore the correlation between fat mass and obesity-associated gene FTO rs9939609 polymorphism and glucose abnormalities and glycometabolism related indexes by comparing the allelic and genotypic frequency. Furthermore, we analysised the sensibility of effects with FTO rs9939609 polymorphism and physical activity for reducing the risk of glucose abnormalities, which could provide a theoretical basis for the long-term aerobic exercise elderly to seek reasonable physical activity recommendations from the perspective of gene polymorphism.MethodRecruited the long-term aerobic exercise elderly from the community of Shanghai,China. Inclusive criteria:(1) BMI≧18.5kg/m2, body weight kept relatively stable over the past six months;(2)≧3 years’ aerobic exercise;(3)≧3 times per week,≧ 30 minutes per time;(4)No hypoglycemic agents drug taken, and no serious cardiovascular disease, such as heart disease. By the initial screening questionnaire and further physical examination, we finally recruited 323 subjects. After removing 2information missing cases, the actual effective subjects were 321 cases, 136 males and185 females, average age of 63.42±3.14 years(60-69). According to China guideline for type 2 diabetes(2013), the subjects were allocated into Glu abnormal group and Glu normal group. Anthropometric, biochemical variables and physical activity by IPAQ were examined in total subjects. FTO rs9939609 polymorphism was genotyped by MALDI-TOF.Results1. In this study, a total of 321 subjects were successfully performed FTO rs9939609 SNP genotyping. Mass spectrometry analysis showed that there were 5 AA genotype, 77 TA genotype and 239 TT genotype. The frequency of A allele distribution was 13.55%, and 86.45% of T allele. All genotype groups obeyed Hardy-Weinberg equilibrium(c2=0.182, P=0.669>0.05).2. After grouping the subjects according to BMI, A allele(16.46% vs 10.51%)and the AA+AT genotype(30.49% vs 20.38%) in the overweight and obesity group were significantly higher than the nomal weight group(P=0.038, 0.028<0.05).Compared with carries of T allele, carries of A allele had a higher risk of overweight and obesity(OR=1.678, 95%CI: 1.055-2.669).3. After grouping the subjects according to FBG, no significant difference was detected in A allele(12.63% vs 14.96%) or the AA+AT genotype(24.22% vs 27.56%)between the Glu abnormal group and the nomal weight group(P=0.503, 0.399>0.05).Stratification by gender, revealed negative association between FTO rs9939609 and glucose abnormalities.4. In Glu abnormal group, the AA+AT genotype subjects had higher BMI and leptin than the TT genotype subjects(P=0.038, 0.047<0.05). The association still existed after adjustment for gender and age.5. With the rising level of physical activity, FBG in AA+AT genotype subjects were decreasing and the difference was significant between subjects whose physical activity level >6153MET-min/w and ≤4176MET-min/w(P<0.05). However, FBG in TT genotype subjects were just slightly decreasing,with no significant difference(P>0.05). FBG had a significant difference between AA+AT genotype subjects and TT genotype subjects when physical activity level reached 6153MET-min/w and above(P<0.05).ConclusionDifferent FTO rs9939609 genotype subjects had different sensibilities for reducing the risk of glucose abnormalities through physical activity. Compared with the TT genotype subjects, the AA+AT genotype subjects were more suitable to reduce the risk of glucose abnormalities with a relatively high level of physical activity(PA>6153MET-min/w).