Purification,Characterization And Preparation Of Anti-Alcohol Liver Injury Component Of Coriolus Versicolor Intracellular Polysaccharopeptide

Alcohol liver disease(ALD) is one of the toxic liver damage by long-term heavy drinking. With social development and people’s living standards improvement, global wine consumption increased incidence of alcoholic liver disease which becomes an important cause of death. Coriolus versicolor intracellular polysaccharopeptide is a kind of protein polysaccharide which has a lot of bioactivities and was mainly involved in anti-chemical and immunological liver injury. However, anti-alcohol-induced liver injury is rarely reported. The active ingredient of C. versicolor intracellular polysaccharopeptide is not clear, the structural properties is uncertain and the extraction process could be improved. This work focused on the anti-alcohol liver activity ingredient of C. versicolor intracellular polysaccharopeptide and the purified active ingredient’s structure information. The main results are as follows.(1) The C. versicolor mycelia was prepared by large-scaled fermentation and the polysaccharopeptide was extracted by hot water extraction and divided into 3 ingredients by step-alcohol precipitation, PSK-1, PSK-2 and PSK-3. And then evaluated the anti-alcohol liver injury activites through the NIAAA mouse model. The results showed that PSK-1 can significantly reduce serum AST, ALT activities, decrease contents of serum Triglycerid, MDA, NEFA, TC and LDL-TC and increase content of serum HDL-TC.(2) PSK-1 was purified through Hiprep DEAE FF column and Superdex 75 column. Two fractions were obtained which named PSK-1b1 and PSK-1c1. The Mw of PSK-1b1 was 21.69 k Da. PSK-1b1 was composed of fucose, mannose, glucose, xylose, galactose and glucuronide. The proposed backbone of PSK-1b1 was â†'1-β-Galp-(6â†'1)-β-Glcp-(4â†'1)-α-Galp-(3â†'1)-α-Manp-(2â†'1)-β-Glcp-4â†'. The Mw of PSK-1c1 was 39.18 k Da. PSK-1c1 was composed of mannose, glucose, xylose, galactose, galacturonic acid and glucuronide. The proposed backbone of PSK-1c1 was â†'1-β-Glcp-(4â†'1)-α-Glcp-(4â†'1)-β-Glcp-(4â†'1)-β-Galp-6â†'.(3) The extraction yields of C. versicolor intracellular polysaccharopeptide(PSK) were compared by high-pressure homogenization(DHPH), hot water extraction(HWE) and ultrasound-assisted extraction(UAE). The yield of PSK by DHPH was 28.33%, which was 2 times higher than HWE. The Mw of DHPH-extracted PSK was a little smaller than HWE-treated PSK. The monosaccharide composition and content were nearly the same by the three methods. The primary structures of PSK were also unchanged. Proportions of the three components extracted by 3 different methods were consistent by ethanol precipitation using 43%, 50% and 80% ethanol respectively. The content of the ingredient with the best anti-alcohol liver injury(PSK-1) was 34.12%, and were 30.74% and 31.29% in DHPH-extacted PSK and UAE-extracted PSK.