Polysaccharopeptide Of Coriolus Versicolor Induced Apoptosis In Leukemia Cells Related To The Death Receptor Pathway

In this study, the regulation of death receptor signal transduction pathway induced by PSP was investigated in HL-60, Molt-4 and Jurkat human leukemia cell lines. MTT assay was used to detect the dose-effect of inhibition of proliferation. Annexin-V assay and morphology observation were applied to identify cell apoptosis. Flow cytometry was used to analyze cell cycle disturbed by PSP treatment, and western blot and flow cytometry techniques were used to investigate the change of the key molecules in death receptor signal transduction pathway.The results showed the growing of human leukemia cells was inhibited by PSP and was related to induction of apoptosis. PSP treatment also led to S phase accumulation in Molt-4 cells. In human leukemia cells including of Molt-4, HL-60, Jurkat, caspase-8 was activated and Fas expression was up-regulated, while TRAIL expression remained unchanged. FADD expression was up-regulated in Jurkat cell while it remained unchanged in Molt-4 and HL-60.These findings imply that PSP induced apoptosis in leukemia cells may be related to Fas signaling in death receptor pathway but not related to TRAIL signaling.