Aloe Emodin Reduces Bone Loss Of Model Mice By Inhibiting Osteoclastogenesis

Background and Objective:The present study found that with the aging of our population, domestic osteoporosis-related fracture incidence rate has increased year by year.osteoporosiss is a kind of characterized by bone mass loss and bone microarchitecture destruction of the diseas. Due to the destruction of the bone and bone loss, osteoporosis incidence of fracture and fractures in patients with delayed union rate is much higher than normal.More and more evidence that osteoporosis is associated with the osteoclast resorption enhanced.Thus, suppression ostoclastic bone resorption, which prevent osteolysis, is a potential method for the treatment of osteoporosis. aloe-emodin is abundant in the rhizome of the Polygonaceae plant which has been used in traditional Chinese medicine. In this study, we found that aloe emodin can reduces bone loss of model mice by inhibiting osteoclastogenesis.Methods:In vitro:To test the effect of the aloe-emodin on RANKL induce-osteoclastgenesis by dose-dependent experiments. Test the effect of the aloe-emodin on bone resorption by bone resorption experiments, PCR and Luciferase report. Toxicity testing of aloe-emodin though MTT experiments.In vivo:Thirty 8 weeks C57 mice were randomly divided into to 5 groups sham group, OVX group,OVX+E2 group and OVX+low and high doses of AE group. Mice were treated intraperitoneally with AE or NS every two days for 6 weeks.The tibia were collected for further examination including micro-computed (micro-CT) tomography.Twenty-four 8 weeks C57 mice were randomly divided into to 4 groups sham group, LPS group, and OVX+ low and high doses of AE group. Mice were treated with AE or NS for 8 days. The calvaria were collected for further examination including micro-computed (micro-CT) tomography.Results:In vitro:Osteoclast proliferation and function can be inhibit by aloe-emodin.0-10 μM of aloe emodin has no toxic effect on osteoclasts.In vivo:Aloe emodin prevented tibia and calvaria bone loss.Conclusions:Our results indicate that aloe emodin has the potential of promoting bone formation and reducing bone loss of model mice.