Effects Of Ultrasonic Phoresis Fufang Sanqi Xiaotong Ointment On The Expression Of Chondrocytes Caspase-3,Caspase-9 And XIAP On Rabbits Knee Osteoarthritis

ObjectiveTo study the effect and change of ultrasonic phoresis fufang sanqi xiaotong ointment outside treatment on rabbits knee osteoarthritis, and the influence on chondrocytes caspase-3, caspase-9 and XIAP expression.MethodsForty New Zealand white rabbits (six months old) were randomly divided into 5 groups: phonophoresis group, ultrasound group, ointment group, model group and normal group,each group of eight. In addition to the normal group, the other four groups of animals were received anterior cruciate ligament amputation (ACLT) to make the rabbit models of KOA, and the intervention carried out after six weeks.The phonophoresis group and ultrasound group used ultrasonic therapy equipment to treatment, by used the intensity of 300 mW/cm2, pulse on-off ratio is 20% and frequency of 1 MHz.The ointment group and model group also used ultrasonic therapy equipment to treatment, but the instrument is not plugged. Treatment once a day,10 minutes at a time, and six days’ treament is a course, there are two courses totally. Remove the femoral condyle cartilage after two weeks’ intervention. Hematoxylin-eosin(HE) staining was conducted for histological observation of rabbit articular cartilage, and the cartilage structure were assessed and quantified with the modified Mankin rating criteria, immunohistochemistry were used to assess the expressions of caspase-3, caspase-9 and XIAP in rabbit chondrocytes.Results1. The general macroscopic observation:comparing with normal group,the treated groups and model group showed different degree of osteoarthritis.The phonophoresis group, ultrasound group and ointment group showed lighter rabbit knee osteoarthritis cartilage damage compared with the model group.2. On the histomorphological,the degree of cartilage degeneration from light to heavy followed normal group, phonophoresis group,ointment group,ultrasound group,modelgroup.3. IOD comparison:Comparing with the normal group, the treated groups and mod el group showed higher expression level of caspase-3,caspase-9 and lower expression 1 evel of XIAP (P=0.000<0.01,P=0.01<0.01,P=0.000<0.01);comparing with the model gro up,the treated groups showed lower expression level of caspase-3,caspase-9 and higher expression level of XIAP (P=0.000<0.01,P=0.01<0.01,P=0.000<0.01);comparing with th ephonophoresis group,the ultrasound group and ointment group showed higher expressi on level of caspase-3,caspase-9 and lower expression level of XIAP (P=0.000<0.01,P= 0.01<0.01,P=0.000<0.01);The ointment group showed lower expression level of caspase-3,caspase-9 and higher expression level of XIAP than the ultrasound group,but there was not statistical significant between them (P=0.991>0.05,P=0.999>0.05,P=1.000>0.0 5).Conclusion1. Fufang sanqi xiaotong ointment phonophoresis, ultrasound and fufang sanqi xiaotong ointmentoutside treatment can effectively delay the rabbit knee osteoarthritis cartilage damage, and fufang sanqi xiaotong ointment phonophoresis has a better curative effect than ultrasound therapy alone or fufang sanqi xiaotong ointment outside treatment.2. The therapeutic mechanism of fufang sanqi xiaotong ointment phonophoresis, ul trasound and fufang sanqi xiaotong ointment outside treatment may be by inhibiting th e caspase-3,caspase-9 expressing and enhancing XIAP expressingof rabbit chondrocytes.