Extraction Of Ldesia Palycarpa Fruit Oil And Seed Oil And Anti-inflammatory Activity Of Their Remains

Taking Ldesia palycarpa as raw material, this paper described a method to extract seed oil and fruit oil making use of organic solvent with the aid of enzyme. In the meanwhile, each extraction factor in the process of seed oil extraction was discussed. Based on the single-factor experiment, the extraction technique was optimized by response surface methodology and then it was verified according to optimized conditions. Polyphenol was distilled from the remaining seed and pods after the oil extraction and its anti-inflammatory activity was tested by both anti-inflammatory in vivo and in vitro anti-inflammatory. Summaries as below:In the single-factor experiment, the pH value, reaction time (min) and reaction temperature (℃) were selected as main study factors, in which case the oil yield was compared. And it was optimized by response surface according to the results of oil extraction under various factors. The optimal extraction technique was achieved after three consecutive experiments with:seed oil:this produced an extraction rate of 11.50%; And with fruit oil:the average oil extraction rate reached 15.46%. The results matched the regression equation quite well, which proved the condition of extraction reliable.Seed oil and fruit oil were treated by methyl esterification methods. And each main component was tested by GC/MS. Seed oil is composed of six main ingredients:Myristic acid, Trans-palmitoleic acid, Palmitic acid, linoleic acid, Oleic acid and Stearic acid. In terms of fruit oil, it consists of seven major oleic acids:Trans-palmitoleic acid, Palmitic acid, linoleic acid, Oleic acid, Stearic acid, Decanoic Acid and Dodecanoic acid.Polyphenol components were distilled from defatted seed and pods in the circumstance of acidified methanol. Selecting Gallic acid as standard sample, the results showed that the concentration of seed polyphenol (SPP) and fruit polyphenol (FPP) were 4.74mg/g and 6.60mg/g respectively. Then cell viability and NO emission were tested after administration of LSP-induced RAW264.7 cells. It suggested that when the concentration of drug administration was during 50～200μg/mL, the value of cell viability increased with the increasing concentration of administration. When the content of nitrite in the call decreased, so did NO emission. The concentration of drug administration caused no side effects on cells and therefore this concentration could be used as a reference to the role of anti-inflammatory cytokine gene expression. After confirmation of the anti-inflammatory effect of SPP and FPP, the genetic expression was justified by the brightness of gel electrophoresis image according to semi-quantitative RT-PCR detective methodology. The results showed that the higher doses of SPP and FPP had obvious impacts on iNOS, IL-6 and TNF-a.After injecting 0.2% carrageenan into rat paws, findings it was that SPP and FPP caused the inhibition of acute inflammation to some extent by comparisons among SPP, FPP, the positive control group and the model group.