Effect Of IL-18 And HIF-1α In Serum And Gingival Tissues Of Rats With Periodontitis Exposed To Chronic Intermittent Hypoxia

Background:Obstructive sleep apnea-hypopnea syndrome is a high incidence, potentially dangerous diseases, which is characterized by multidisciplinary cross diagnosis and treatment.Because of its associated with many diseases, therefore become the research hot spot.Chronic intermittent hypoxia is the main pathological characteristics of OSAHS,chronic intermittent hypoxia by oxidative stress reactions in the body, to form the body of systemic inflammation. And periodontitis is an common disease. There are epidemiological investigation showed that OSAHS have significant relationship with the happen of periodontitis.At present there are not many research for OSAHS and perodontitis.We through the establishment of periodontitis rats model in the case of chronic intermittent hypoxia, detecting chronic intermittent hypoxia on IL- 18、HIF-1α,and analyse the possible mechanisms among OSAHS and periodontitis.Methods:32 SD rats were randomly divided into 4 groups, A: normoxia control group(Regular feeding); B: normoxia periodontitis group(ligating the bilateral maxillary second molar); C: intermittent hypoxia control group(under the CIH condition mimicking OSAHS, 8h per day); D: intermittent hypoxia periodontitis group(treatment with group B + C). Experimental period for 8 weeks. ALL SD rats were sacrificed in end of the experiment. The indexes of periodontal were detected. The concentration of IL –18, HIF-1α in periodontitis tissue and serums were measured using enzyme linked immunosorbent assay(ELISA), and analyse the correlation with perodontal index.Results:1.After 8 weeks,B group and C group than the A group compared to AL were significantly increased(P < 0.05); group than the B and C compared to AL were signifantly increased(P<0.05).2.D group is the most severe destruction of alveoar bone in 4 group.Radiograpgy demonstrated the exposure of root furcation and accompanied by vertical bone resorption.3.Compared with A group,the levels of IL-18、HIF-1α in B and C group increased(P< 0.05); Compared with D group, the levels of IL-18 、 HIF-1α in B and C group decreased(P<0.05).4.This study AL, IL-18 、 HIF-1α in D group comparisons between the positive correlation: AL and IL-18 compared positively correlated(P < 0.05); AL and HIF-1αcompared positively correlated(P<0.05).Conclusion:Under the condition of CIH in this experiment, periodontitis rats model were successful established; Chronic intermittent hypoxia can lead AL increased in rats, which is correlated with the expression of IL-18 and HIF-1α in serum and gingival tissues.