| Objective :The aim of this study is to reveal the Xinjiang HLA-DRB1, DQB1, DPB1 gene polymorphism, elucidate the correlation of DRB1, DQB1, DPB1 and pulmonary tuberculosis by PCR-SBT method on HLA-DRB1, DQB1 and DPB1 in Xinjiang Han pulmonary tuberculosis patients and healthy individuals.Methods: We used polymerase chain reaction and direct gene sequencing typing(PCR-SBT) for genotyping of HLA-DRB1, DQB1 and DPB1 in 92 cases of Han pulmonary tuberculosis patients and 96 cases of Han healthy individuals, respectively, in Xinjiang uygur autonomous region. The gene frequencies were calculated by direct counting method, and the haplotype frequencies and Hardy-Weinberg equilibrium calculation were obtained by using the maximum likelihood method by using Arlequin3.5 software. The comparison between the groups would use the chi-square test. OR value and 95% confidence interval was used to evaluate the risk of tuberculosis.Results:1. The HLA-DRB1,DQB1 and DPB1 three locus in case group and control group were balanced by Hardy-Weinberg equilibrium test.2. we observed 30 HLA-DRB1 alleles in pulmonary tuberculosis patients and 29 HLA-DRB1 alleles in healthy controls. The frequencies of HLA-DRB1 * 07:01(15.8%vs8.3%, OR=2.058, P=0.027)is significantly higher in pulmonary tuberculosis patients than those in healthy controls, the difference was statistically significant.3. We observed 16 HLA-DQB1 alleles in pulmonary tuberculosis patients and 29 HLA-DQB1 alleles in healthy controls.The frequencies of HLA-DQB1 * 03:01(28.3%vs13.5%, OR=2.515, P=0.0004)is significantly higher in pulmonary tuberculosis patients than those in healthy controls, the difference was statistically significant.4. We observed 12 HLA-DPB1 alleles in pulmonary tuberculosis patients and 16 HLA-DPB1 alleles in healthy controls.The frequencies of HLA-DPB1 * 05:01(37.5%vs28.1%, OR=1.554, P=0.049)is significantly higher in pulmonary tuberculosis patients than those in healthy controls, the frequencies of HLA-DPB1*02:01(16.8%vs27.6%, OR=0.552, P=0.010)is significantly decreased in pulmonary tuberculosis patients than those in healthy controls, the differences were statistically significant.5. We observed 43 DRB1-DQB1 haplotypes in pulmonary tuberculosis patients and 98 DRB1-DQB1 haplotypes in healthy controls. The frequencies of DRB1 * 07:01-DQB1 * 02:02(10.9% vs 4.7%, OR=2.480, P=0.025), DRB1 * 09:01-DQB1 * 03:03(10.9% vs 5.2%, OR=2.220, P=0.043) haplotypes is significantly higher in pulmonary tuberculosis patients than those in healthy controls, the differences were statistically significant.6. We observed 76 DRB1-DPB1 haplotypes in pulmonary tuberculosis patients and 81 DRB1-DPB1 haplotypes in healthy controls. There was no significant difference in haplotype frequencies between the two groups.7. We observed 56 DQB1-DPB1 haplotypes in pulmonary tuberculosis patients and 83 DQB1-DPB1 haplotypes in healthy controls. The frequencies of DQB1*03:01-DPB1*05:01(15.8% vs 7.5%, OR=2.379, P=0.010) and DQB1*03:03-DPB1*05:01(19.8% vs 2.3%, OR=4.055, P=0.004) haplotypes is significantly higher in pulmonary tuberculosis patients than those in healthy controls, the differences were statistically significant.8. We observed 86 DRB1-DQB1-DPB1 haplotypes in pulmonary tuberculosis patients and 135 DRB1- DQB1-DPB1 haplotypes in healthy controls. The frequencies of DRB1*11:01-DQB1*03:01-DPB1*05:01(5.4% vs 1%, OR=5.869, P=0.015) and DRB1*09:01-DQB1*03:03-DPB1*05:01(8.7% vs 1.6%, OR=9.964, P=0.002) haplotypes is significantly higher in pulmonary tuberculosis patients than those in healthy controls, the differences were statistically significant.Conclusion:1.HLA-DRB1, DQB1 and DPB1 exist rich polymorphism in Han nationality in xinjiang.2.The results suggest that HLA-DRB1 * 0701, DQB1*03:01 and DPB1*05:01 may be the susceptible factors to pulmonary tuberculosis, DPB1*02:01 may be the protective factors to pulmonary tuberculosis. DRB1 * 07:01-DQB1 * 02:02, DRB1 * 09:01-DQB1 * 03:03, DQB1*03:01-DPB1*05:01, DQB1*03:03-DPB1*05:01, DBR1*11:01-DQB1*03:01-DPB1*05:01, DRB1*09:01-DQB1*03:03-DPB1*05:01 haplotypes may be susceptible to pulmonary tuberculosis in Han population from the Xinjiang uygur autonomous region of China. |
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