| Objective: To prepare the porous drug loaded PLGA/chitosan/nano-hydroxyapatite membrane for the guided periodontal tissue regeneration in surgical implantation, and characterize its physical and chemical properties, meanwhile to preliminary explore its cell compatibility.Materials and Methods: Prepared different ratio of PLGA and CS(100/0, 90/10, 80/20, 100/0) of the PLGA/CS/nHA composite membrane by freeze-drying method whit polyvinylpyrrolidone as porogen. And detected composite membrane porosity by anhydrous ethanol liquid displacement method, water absorption by ratio of wet and dry weight of the film, wet mechanical performance by electronic universal material testing machine, degradation by mass loss method. And then selected the optimal proportion and analyzed the structure and crystalline state by Fourier Transform Infrared Spectrometry(FTIR) and x-ray diffractometer. Prepared clindamycin sustained-release film and detected its drug release by Uv spectrophotometry, proliferation of the periodontal ligament fibroblasts(PLFs) by CCK 8 and scanning electron microscope.Result: When the ratio of PLGA/CS was 90/10, the properties of drug-loaded PLGA/CS/nHA composite membrane were the best. Its porosity was(28.66±1.35)%,water absorption was(108.65±2.27)%, tensile strength was(2.36±0.04)MPa, elongation at break of films was(203.64±3.89)%, breaking power was(45.98±2.46)N and degradation was(17.60±0.86)%. The drug released effective concentration for 15 d and the membrane can promote the proliferation of PLFs.Conclusion: The porous PLGA/chitosan/nano-hydroxyapatite films had optimal porosity, and it was accepted that the material degradation in vitro with respect to tissue growth, created and maintained a specific space to guided periodontal tissue regeneration, released drug steady in a certain time, and were valuable to be further studied for experiment in vivo. |
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