| Periodontal disease is a common chronic disease of periodontal tissues. Because of its slow onset, it is not easy to arouse people’s attention in its early stage. With the development of the disease, people come to pay attention to it in advanced stage,which means people miss the valuable opportunity of treatment, and are the main factor that results in teeth loss of older people. Guided Periodontal Tissue Regeneration(GPTR) is a focus technology raised in recent years by setting an artificial composite membrane at the exposed root to prevent gingival epithelial cells and fibrous connective tissue from adhering to the root surface; and at the same time,to guide the periodontal ligament cells enriched at the exposed root surface and differentiate into new cells for periodontal tissue regeneration in order to form new periodontal tissue, and to achieve the purpose of periodontal tissue regeneration.Nowadays, it cannot be achieved to restore normal periodontal tissue structure and get satisfactory results by common clinical treatment method. In recent years, periodontal regeneration therapy has become a new hot spot for the treatment of periodontal disease. With the continuous development of biomedical materials, it is expected that the newly synthesized materials will be applied to the regeneration of periodontal tissues.Objective : In this study, PLGA/CS/n HA composite membrane is prepared by freeze-drying method, and combined with different types of cells, and then they are observed under electron microscope and biological compatibility experiment is conducted. The biocompatibility of PLGA/CS/n HA composite membrane will be discussed and this study will provide scientific experimental basis and theoretical basis for the clinical research of novel materials of guided periodontal tissue regeneration(GPTR).Methods: The adhesion of periodontal ligament fibroblasts were observed on 4 kinds of materials( C0, C10, C20, C30) through cell adhesion assay; the proliferation of periodontal ligament fibroblasts were tested on C0, C10, C20, C30 by cell proliferation CCK-8 test; gene expression of factors of FGF-2, Periostin, TGF-β1were tested on these materials by RT-PCR experiment; osteogenic differentiation of mesenchymal stem cells were observed on these materials by alizarin red experiment and alkaline phosphatase experiment.Results: The adhesion of periodontal ligament fibroblasts were in good condition, and the condition of C10 group was best. In cell proliferation CCK-8 test, the condition of C10 group was best, and on every time point, cell densities of C10 group and other 3group were contracted: it was 1.6, 1.4 and 1.3 times than cell densities of C0,C20,C30 groups respectively on day 1(P<0.01); and 1.4, 1.3, 1.2 times on day4(P<0.01); 1.3, 1.2, 1.1 times on day 7(P<0.01). There was gene expression of factors of FGF-2, Periostin, TGF-β1 in these materials, and the highest expression was found in C10 group, however, gene expression of FGF-2 was near on 4 kinds of material,and there was a big gap of gene expression of TGF-β1. Alizarin red experiment showed calcium nodules were the most in C10 group. In alkaline phosphatase experiment, alkaline phosphatase activity was the highest in C10 group up to18.1U/10 ml on day 7.Conclusion: The PLGA/CS/nHA composite membrane has good biocompatibility,and can work well with cell adhesion, promote cell proliferation and differentiation. |
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