| Objective: In Bilateral breast cancer, According to the occurrence time or diameter,of tumors can be divided into Starting cancer and Second cancer, the mechanism is not clear at present. In this study, differences were observed in the expression of inhibitors of apoptosis proteins Survivin and P53 mutant gene in bilateral breast cancer in starting and Second cancer, bilateral breast cancer further reveal the molecular point of view of having a single clone(the same genome expression) or independent clones of(independent genome expression),and the correlation between P53 and Survivin in breast cancer.Methods: 10 cases of women with bilateral breast cancer patients from affiliated hospital of Zunyi Medical College was collected by a number of pathologist histologically confirmed, the selected object of study tissue samples and clinical data integrity, before the first surgery did not receive neoadjuvant chemotherapy. 10 patients diagnosed with unilateral breast cancer patients as the control group. Using tissue microarray-based immunohistochemical Max Vision Survivin and P53 were detected two molecular markers in their first bilateral breast cancer group, the second group of cancer and contrast with unilateral breast cancer in three groups specific expression. 5 high-power field using Image-pro Plus6.0(IPP) image analysis software to interpret the results of the slice, each slice select a representative, IPP analysis software sliced accumulated optical density(IOD Sum), select the entire slice area as(area), determined to calculate the average optical density(mean density), as a quantitative expression of the molecule on each slice markers.Using this method, a quantitative analysis of molecular markers in two experimental and control groups, respectively, in the expression. Data processing using statistical software SPSS20.0 sample normality test, homogeneity of variance test, and ANOVA to compare the expression of Survivin and P53 among the three groups; the use of correlation and regression analysis and comparison of P53 and Survivin in expression of breast cancers.Results: In Bilateral breast cancer, P53 in the starting cancer, cancer foci in the second and unilateral breast cancer case were expressed as a percentage(11.911 ± 9.555,6.460 ±5.037,14.580 ± 13.763), the expression between starting cancer and second cancer was no difference(p=0.230> 0.05);it was no differences between starting cancer and unilateral breast cancer(p=0.370>0.05); it was a relatively significant difference between the second cancer and unilateral breast cancer(p=0.045<0.05).Survivin cancer in first, second and unilateral breast cancer in the case were expressed as a percentage(24.390 ±11.672,26.319 ± 11.473,21.815 ± 12.355); it was no significant difference between starting cance and the second cancer(p=0.718>0.05), there was no statistically significant difference between starting cancer and second cancer,unilateral breast cancer(p=0.631>0.05,p=0.402>0.05). P53 and Survivin expression reached a low negative correlation in the tables of breast cancer(γ =-0.34, p =0.033).Conclusion: Expression of P53 and Survivin instarting cancer and second cancer was no statistically different in bilateral breast cancer, it related that the starting cancer and the second cancer occurs might have the same genes. it was no significant difference between starting cancer and unilateral breast cancer. The expression of Survivin in the second breast cancer and unilateral difference was no different, P53 were significantly different in comparison with a unilateral expression of a second breast cancer, suggesting the possibility of other therapeutic factors can also affect the expression of results. Expression of P53 and Survivin in breast cancer have a low degree of negative correlation. |
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