MiR-370 Inhibit The Proliferation And Migrate On Glioma

Micro RNA is a class of small non-coding RNA molecules with the function of regulating, recent studies have shown that mi RNA can regulate gene expression in a post-transcription level. Abnormal mi RNA expression is closely related to the occurance and development of glioma. Although the mechanism of some abnormal expression of mi RNAs has been interpreted, but there is no report about the research of mi R-370 in glioma.To explore the role of mi R-370 in glioma, the study detected and analyzed the expression of mi R-370 in glioma tissue samples and glioma cell lines using quantitative real-time PCR technique, the results showed that mi R-370 expression in a majority of glioma tissue is lower than that in normal tissue,and mi R-370 expression in SHG44, U87 and U251 glioma cells showed various degrees of decline compared with that in normal brain tissue. The result of MTT proliferation assay showed that mi R-370 overexpression can inhibit proliferation of glioma cells, on the contrary, Inhibiting the expression of mi R-370 can promote the proliferation of glioma cells. To further explore the mechanism of mi R-370,using the PI/Annexin V staining technique to analyze cell apoptosis by flow cytometry, and it was found that mi R-370 overexpression promoted the apoptosis of glioma cells. In addition, PI staining analysis indicated that mi R-370 showed no significant effect on each time of glioma cell cycle. The result of cell migration showed that mi R-370 overexpression can inhibit the migration of glioma cells, while in contrast, inhibition of mi R-370 expression promoted the migration of glioma cells. Through bioinformatics software analysis we found DNMT3 A may be target gene of mi R-370,and mi R-370 regulated the expression of DNMT3 A in m RNA level and protein levels by quantitative real-time PCR and Western blot analysis, and dual luciferase reporter assay confirmed that DNMT3 A was the target gene of mi R-370.In summary, mi R-370 can inhibit the proliferation and migration of glioma cell, and DNMT3 A acted as the target gene of mi R-370.