| Objective:To Study the extraction process and purification process of flavonoids of Sarcandra glabra.Method:With the measurement of flavonoids、Isofraxidin and extracting ratio as index, the optimum extraction process was selected with the orthogonal design method.With the measurement of flavonoids、Isofraxidin and extracting ratio as index, the first process of optimum purification condition comfirmed by uniformity design. During the reseach of purification with rosin,with the ratio of mobile-absorbing and separating as index,The optimum parameters of purification were determined by singal factor. Using the content of flavonoids as a index, the effect of different temperature was contrasted after vacuum drying.The quality standard of Sarcandra glabra was studied by TLC、visible spectrophotometry and HPLC.Result:(1) extraction process Sarcandra glabra was crushed into rough powder,2 times of refluxing with water,14、12 times of water-adding amount and 2.5,2.0 hours of refluxing time respectinely. (2) purification process The alcohol method was Comfirmed to use to flocculate the water solvent and the optimum purification condition was:relative density of concentrate was 0.6(meterials vs extracting solution),the percent of alcohol was 50%,the temperature was 20℃. The optimum parameters of purification was:sample concentration was 0.6g·ml-1,sample flow velocity was 2BV·h-1,then being eluted with volume of 4 BV of water, volume of 4 BV of 40% Etoh at the velocity of 16BV·h-1 gathering the part eluted by 40% Etoh. (3) study of concentration and desiccation The optimum temperature was 60℃. (4) Study of Quality Standards Visible spectrophotometry and HPLC method was established for the determination,the purity of flavonoids extract was 82.38%,The average recovery rate was 97.4%,while RSD was 1.23%.Conclusion:Process was reasonable,reliable,accurate and provided a scientific and rational,practical basis for the development of Chinese herbal medicine study. |
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