Isolation And Identification Of Microorganisms In Waterlogged Wood And Lacquer Stored In Ancient Chu Tomb No.1 At Tianxingguan In Jingzhou

Microbial damage is an important disease on waterlogged wood and lacquer and also a key factor leading waterlogged wood and lacquer to continue deteriorate and even disappeared. In the process of tomb storage, digging, cleaning, cultural relics Of wood and lacquer are likely affected by microbial contamination. Unearthed wood and lacquer continue to be kept in clean water. The chemical state and physical properties of wood and lacquer soaked in water for a long time are degraded and eroded by bacteria, making cultural relics could not complete show their original appearance or directly affected the full representation of archaeological information. Isolating and identifying microbes in waterlogged wood and lacquer is of great significance for recognizing the microbial corrosion law of wood and lacquer and studying microbial corrosion inhibition method of waterlogged wood and lacquer, thus reducing the decay and deterioration of f waterlogged wood and lacquer and prolonging the preservation time.This paper studied the colored drawing wood and lacquer and the environmental water sample stored in waterlogged pool in basement in Jingzhou museum for recent 30 years. The colored drawing wood and lacquer were unearthed from ancient Chu Tomb No.l at Tianxingguan in Jingzhou in 1978. Select part of three representative colored drawing wood and lacquer and the environmental water as samples. Separate and cultivate microbial, research the amount and type of microorganism of waterlogged wood and lacquer, investigate the presence of microbes in waterlogged wood and lacquer:the amount of contaminated microorganism in the three colored drawing wood and lacquer samples were different, they were lower than the number of microorganisms in water environment, among them priority was given to bacteria, which was changed in the range of 240-240 CFU/g; Followed by fungus, changed in the range of 0-90 CFU/g; actinomycetes were fewest even not detected; Bacteria in the water environment of 1590 CFU/L, fungus of 1040 CFU/L, actinomycetes not detected; In view of the main pollution microbes -- bacteria, the traditional morphological, physiological and biochemical identification methods and modern molecular identification techniques were used to identified the isolated bacterial and 46 species were obtained. Results of this study:In F455, no.1 and no.28 are Bacillus thioparans, no.2 is Bacillus idriensis, no.5 is Bacillus halmapalus, no.9 and no.10 are Bacillus pumilus, no.14 is Bacillus pseudomycoides, no.17, no.19 and no.76 are Bacillus cereus, no.23 is Brevundimonas diminuta, no.29 and no.51 are Bacillus thuringiensis, no.36 is Brevibacterium epidermidis, no.50 is Alcaligenes faecalis subsp, phenolicus. In F446, no.1, no.9,no.10,no.11,no.19 and no.22 are Bacillus cereus, no.6 and no.20 are Brevibacterium epidermidis, no.8 is Lysinibacillus fusiformis, no.14 is Alcaligenes faecalis subsp,phenolicus, no.16 is Bacillus pseudomycoides, no.25 is Bacillus thuringiensis. In F452, no.1, no.2and no.7 are Bacillus cereus, no.6 is Bacillus thuringiensis. In F water, no.1 is Bacillus thuringiensis, no.4 is Altererythrobacter atlanticus, no.5, no.23, no.27 and no.51 are Brevibacterium epidermidis, no.7 is Alcaligenes faecalis subsp. Phenolicus. No.17 is Flavobacterium mizutaii. No.19 is Paenibacillus dendritiformis.no.32 and no.35 are Brevundimonas diminuta. No.43 is Bacillus cereus. No.46 is Ochrobactrum pseudogrignonense.Preliminary identificated strain using glycerol at 4°c refrigerator for later test.