The Effects Of Sport Intervention On Type2Diabetic Rat Liver Tissue’s AKT-GSK3Signal Transduction

Objective: This study investigated the possibility mechanism of glucose metabolism disorder intype2diabetic rat liver and clarified the exercise intervention affect and mechanism of liverdisorder of glucose metabolism in type2diabetic rats.Methods: The15-month-old male SD rats used in this experiment were randomly divided intofour groups----control group (CON), model group (DC), diabetic chronic exercise group (DCE),and diabetes acute exercise group (DAE). Excpect of CON group provided with normal diet, theother three groups gave high fat diet to for eight weeks, and then, intraperitoneal injection ofSTZ of small doses for induced T2M. After the modeling, the group of diabetic chronic exercisegroup (DCE) was applied to the eight-week long exercise intervention, diabetes acute exercisegroup (DAE) was only applied to a one-time exercise intervention while the long-term exercisegroup in the last training. Harvested after the end of the exercise intervention, rapid bloodglucose was measured for fasting glucose and insulin tolerance test(ITT), spectrophotometricmeasurements of rat liver glycogen content. The expression of t-AKT2,t-GSK3α,t-GSK3β andp-AKT2,p-GSK3α, p-GSK3β were detected by Western blotting.Results: Compared with CON group, DC group glycogen content was significantly decreased,after the exercise intervention, the DCE rats’ glycogen content was significantly increased(p<0.05); the protein expression of GSK3α and GSK3β in DC group was significantly increased(p<0.05), while, P-GSK3α and P-GSK3β was significantly decreased (p<0.05); the proteinexpression of GSK3α and GSK3β in DCE group was significantly decreased compared with theDC group (p<0.05); while the expression of P-GSK3α and P-GSK3β protein were elevated (p<0.05). The protein expression of AKT2and P-AKT2in DC rat liver compared with the CONgroup showed a downward trend (p <0.05), AKT2and P-AKT2protein expression of the DCErats with long-term endurance exercise were increased (p <0.05).Conclusion: The impaired AKT2-GSK3signaling pathway might play an important part in thedecline of liver glycogen content of type2diabetic rat, and long-term endurance exercise couldfix AKT2-GSK3signal transduction pathways, contributing to improve the level of glycogen inthe liver, further more, to improve liver glucose metabolism.Recommendation: Continue to study PEPCK and G6P which closely related to gluconeogenesisin liver. Enrich glycogen storage mechanism regulated by GSK3in liver.