The Effect Of Combined Intervention Of Exercise And Metformin For LKB1-AMPK-GLUT4Signal Pathway In The Skeletal Muscle Of Type2Diabetic Rats

Objective: This thesis, on one hand is to discuss whether a six weeks`intervention of swimmingand metformin can make a combined synergistic effect in improving LKB1-AMPK-GLUT4signal pathway in the rats of type2diabetes that induced by the combination fo a high-fat dietand a low-dose STZ, and on the other hand is to provide scientific theoretical basis for clinicaltreatment of type2diabetes.Methods: Fifty-five SD rats was randomly divided into five groups, it were normal controlgroup(NC)、diabetes control group(DC)、diabetes exercise group(DE)、diabetes medicinegroup(DM)、diabetes exercise and medicine group(DEM); The NC was feed with normal dietand all the diabetic groups were feed with high-fat diet; A low-dose STZ was given to all groupsof diabetes to build the model of type2diabetes after seven weeks, and then the DE group wasgiven six week`s swimming intervention, the DM group was given six week`s metforminintervention, the DEM group was given a six week`s combined intervention of swimming andmetformin. After six weeks, the levels of blood glucose、insulin、Kitt、TG、FFA、MG、P-LKB1、P-AMPKa12、AMPKa2、P-AMPKa2、GLUT4in all groups should be tested.Results: Compared with NC, the levels of blood glucose、insulin、TG、FFA are significantlyrising in the DC（P<0.01、P<0.01、P<0.01、P<0.01）, the levels of Kitt、MG、P-LKB1、P-AMPKa12、AMPKa2、P-AMPKa2、GLUT4are significantly descending in DC（P<0.01、P<0.01、P<0.05、P<0.05、P<0.05、P<0.01、P<0.01）; Compared with DC, the levels of insulin、TG、FFA are significantly descending in DE（P<0.05、P<0.05、P<0.05）, the level ofP-AMPKa2、GLUT4is significantly rising in DE（P<0.05、P<0.05），the levels of bloodglucose、insulin、TG、FFA is significantly descending in DM（P<0.05、P<0.05、P<0.05、P<0.05）, the levels of Kitt、MG are significantly rising in DM（P<0.05、P<0.05）, the levelsof blood glucose、insulin、TG、FFA are significantly descending in DEM（P<0.01、P<0.01、P<0.01、P<0.01）, the levels of Kitt、MG、P-LKB1、P-AMPKa12、AMPKa2、P-AMPKa2、GLUT4are significantly rising in DEM （P<0.01、P<0.01、P<0.05、P<0.05、P<0.05、P<0.01、P<0.05）; Compared with DE, the levels of blood glucose is significantly descending in DEM（P<0.05）.Conclusions: The intervention of exercise、 metformin and combination of exercise andmerformin can make a different degrees impact for in improving glucose and lipid metabolism disorder of type2diabetes, the combined intervention of exercise and metformin can effectivelyrepair the injury of LKB1-AMPK-GLUT4signal pathway in the skeletal muscle of type2diabetes, and the intervention of simple exercise can partly repair the injury of AMPK-GLUT4signal pathway, and the intervention of simple metformin make no difference for the injury ofLKB1-AMPK-GLUT4signal pathway.