Effect Of Exercise And Supplement Silk Amino Acids On Cardiac And Skeletal Muscle Antioxidant Capacity In Aging Rats

The 21 st century will be China’s aging population accelerated phase, along with the rapid growth of the elderly population, family, social, medical resources, facing increasingly heavy pressure. Among the many theories concerning the interpretation of aging mechanisms, the free radical theory of aging has been more widely recognized and research, cardiac and skeletal muscle is the largest organ of the human body produces free radicals, also the buffer machine forefront endogenous free radicals, with with age, the amount of free radical production, antioxidant defense system homeostasis and repair oxidative damage functions among system functions are gradually destroyed, the accumulation of oxidative stress on the body leads to irreversible damage of aging. Against free radicals and slow the heart of the most common skeletal muscle aging intervention program is exercise and nutritional supplements. This study of the natural aging rats aerobic exercise training and supplementary silk amino acids, natural aging rats to detect cardiac, skeletal muscle conditions selected indicators, analysis of moderate-intensity aerobic exercise model and silk amino acids to supplement the natural aging rat heart skeletal muscle and influence to further explore the mechanism of action to lay the foundation.Methods: This study selected 14-month-old male SD rats natural aging for 24 subjects were randomly divided into four groups: control group(group C), exercise intervention group(E), supplements the intervention group(X), sports and supplements the intervention group(EX), n = 6. Supplementary schedule of moderate intensity aerobic training and silk amino acids. Sacrificed four weeks after the official campaign, analyzing and comparing each group MDA(malondialdehyde)in cardiac and skeletal muscle homogenates and T-SOD(total superoxide dismutase) activity.Experimental Results:Cardiac T-SOD activity: Compared with group C, E myocardial T- SOD activity was significantly increased(P <0.05), X myocardial T- SOD activity was significant increased(P <0.01), no significant differences EX group; no significant difference in the activity of myocardial T- SOD X group and the group E; E group compared with the group X, significantly reduce myocardial T- SOD activity EX group(P <0.01).Myocardial MDA content: Compared with group C, the content of E myocardial MDA had significantly lower(P <0.05), the content of X myocardial MDA has a very significant reduction(P <0.01), the content of EX myocardial MDA has a very significant reduction(P <0.01); compared with group E, content X myocardial MDA has a very significant reduction(P <0.01), the content of EX myocardial MDA has a very significant reduction(P <0.01); Very significantly lower than the X group P <0.01 EX myocardial content of MDA.T-SOD activity in skeletal muscle: Compared with group C, E group skeletal muscle TSOD activity was significantly increased(P <0.05), no significant differences in X group and EX groups; skeletal muscle X group and EX groups There were no significant differences in T- SOD activity; compared with group E, X skeletal muscle T- SOD activity was significantly lower(P <0.05).MDA content in skeletal muscle: Compared with group C, E group X group, content, EX group of skeletal muscle MDA were significantly lower(P <0.01). Compared with the E group, X group, the MDA content in skeletal muscle EX group there was a significant decrease(P <0.01); skeletal muscle MDA content EX group was significantly lower than the very X group(P <0.01).Conclusion1, surrounded by moderate-intensity aerobic exercise can be very significant increase in rat cardiac and skeletal muscle T- SOD activity of aging, reduce the content of MDA in cardiac and skeletal muscle, reduce body lipid peroxidation damage to the body.2, silk amino acid supplements can be very significant reduction in the content of the aging rat heart and skeletal muscle MDA cardiac and skeletal muscle and reduce body fat content of hydrogen peroxide, reduce cell damage. But the silk amino acids on cardiac and skeletal muscle T- SOD activity causing different effects after a single use of silk amino acids relative to the control group was significantly increased myocardial T- SOD activity, and skeletal muscle T- SOD activity was not significantly changed.3, silk amino acid supplements after exercise to reduce the effect of cardiac and skeletal muscle MDA content in aging rats is very significant, very significant aerobic exercise is better than just silk amino acids and only add effects, a significant interaction between the two, in combination can be used very effectively reduce free radical damage to the body, play anti-aging effects. But after exercise supplement silk amino acids lead to myocardial T- SOD activity was significantly lower than the individual movements and silk amino acids supplemented by a separate case, and skeletal muscle T- SOD activity was not significantly changed. The mechanism for this result requires further experimental analysis.