Effects Of Acute And Four Weeks Resistance Exercise On Antioxidant Capacity Of Aging Skeletal Muscle In Mice

OBJECTIVE: According to the investigation of the changes of muscle antioxidant capacity in aging and young mice during the acute and the long-term resistance exercise,the study aimes to discuss the response of aging skeletal muscle to acute resistance exercise and the changes of aging skeletal muscle in long-term resistance during exercise.As a result, the study preliminarily illustrated the initial physiological mechanism of exercise in anti-aging.METHODS: Thirty-three 3-month-old C57BL / 6 mice were randomly divided into 6 groups: young group (YC), young acute resistance group (YAR), youth long-term resistance group (OCR), old control group (OC), old acute resistance exercise group(OAR), old long - term resistance exercise group (OCR). D-galactose was injected subcutaneously with D-galactose at a dose of 500 mg / kg / d for 8 weeks to develop a subacute aging C57BL / 6 mouse model. The exercise mode was a classic ladder training.In a single acute resistance exercise and four weeks, (T-AOC), total superoxide dismutase (T-SOD), malondialdehyde (MDA) and other indicators of the body weight,total protein content, total antioxidant capacity To detect.Results: (1) Intervention before exercise, body weight of mice in each group was significant difference (P> 0.05) does not exist; different degrees of change in body weight of mice after exercise intervention. Compared with YC group, there was no significant difference (P> 0.05) between YAR group and YC group (P <0.05). The Y group was significantly decreased (P <0.05); OC group was significantly decreased (P<0.01) (P <0.01); OCR group was significantly lower (P <0.05). There was no significant difference between OC group and OAR group (P> 0.05). There was significant difference between OC group and OAR group (P <0.05). Two-factor analysis showed that aging resulted in a significant decrease in body weight (P <0.01), and the effect of resistance exercise on body weight change was not significant (P> 0.05).(2) Compared with YC group, YAR group was significantly increased (P <0.05),YCR group was significantly increased (P <0.01); OC group was significantly decreased(P <0.01); OAR group was significantly reduced ( P <0.01). There was no significant change in OCR group (P> 0.05). Compared with the OC group, there was no significant change in the OAR group (P> 0.05), but showed an increasing trend; OCR group was significantly increased (P <0.01). Two-factor analysis showed that senescence resulted in a significant decrease in T-AOC in the femoral rectus (P <0.01), and resistance to exercise was able to significantly increase the T-AOC (P <0.01).(3) Compared with T-SOD in YC group, the level of YAR group did not change significantly (P> 0.05), but the increase was significant (P <0.05); OC group was significantly decreased (P <0.05) (P> 0.05). There was no significant change in OAR group (P> 0.05). Compared with the OC group, there was no significant difference (P>0.05) in the OAR group, but showed an increasing trend. The OCR group was significantly increased (P <0.01). Two-factor analysis showed that T-SOD increased significantly (P <0.01), and the effect of senescence on T-SOD of femoral rectus was not significant (P> 0.05).(4) Compared with YC group, there was no significant difference (P> 0.05) in YAR group (P> 0.05), but it showed a decreasing trend; YCR group was significantly decreased (P <0.01) (P <0.05). OCR group was significantly lower (P <0.01). Compared with OC group, OAR group and OCR group were significantly decreased (P <0.01).Two-factor analysis showed that senescence could significantly increase the content of MDA in the femoral rectus of mice (P <0.01), while the MDA in the femoral rectus muscle of the anti-resistance mice had significant inhibitory effect (P <0.01)Conclusion:(1) Aging of experimental animals caused by skeletal muscle obvious oxidative stress, leading to lipid peroxidation damage, resulting in the body's antioxidant enzyme activity and antioxidant capacity decreased.(2) Acute resistance exercise can reduce oxidative stress-induced lipid peroxidation level, while the body's oxidase activity and antioxidant capacity changes show a certain degree of inconsistency.(3) Four weeks resistance exercise can significantly improve the antioxidant enzyme activity of aging skeletal muscle, reduce the level of lipid peroxidation, improve the body's antioxidant capacity.