Research Of Incoherent Digital Holographic Microscopic Imaging Based On Michelson Interferometer

Holography is the use of interference and diffraction principle to record and reproduce the real three-dimensional information technology.With the development of photoelectric sensor devices and computer technology,digital holography has been rapid development.Digital holography compared with traditional holography has the characteristics of low production cost,fast imaging speed,record and reproduction flexibility.Non-coherent digital holography The illuminated light source is incoherent and avoids speckle noise caused by coherent light.In recent years,with the continuous efforts of researchers at home and abroad,non-coherent digital holography has been successfully applied to micro-imaging,spectral imaging and telephoto imaging and other fields.In this paper,non-coherent digital holographic microscopic imaging based on Michelson interferometer is studied.The following is the main content of the paper:(1)In this paper,the development of digital holography and non-coherent holography is introduced in detail.The basic theory of optical holography is introduced,and the Fresnel diffraction integral is deduced.Based on the non-coherent digital holographic optical path based on the Michelson interferometer,the point spread function of the incoherent digital holography system based on the Michelson interferometer is deduced.The recording method and the reproducing principle of the non-coherent digital hologram are discussed.The advantages and disadvantages of Fresnel diffraction reconstruction algorithm,convolution diffraction reconstruction algorithm and angular spectrum diffraction reconstruction algorithm.And introduces the method of eliminating zero-order and twin images in non-coherent digital holography.In particular,three-step phase shift technique and generalized three-step phase shift technique are introduced.The feasibility of the imaging system is proved by the hologram and the reconstructed image of the resolution plate and the ruler.(2)Secondly,the non-coherent digital micro-holographic imaging optical path based on Michelson interferometer is constructed,and the system is studied in detail.The point spread function of the system in the process of calculation is calculated,and the concrete expression of the system lateral magnification and reconstruction distance is obtained.Using the CCD hologram,the generalized phase shift digital holographic interferometry was used to remove the twin image and the zero order image,and the reconstructed image was obtained with the angular spectrum algorithm.The non-coherent holographic microscopic imaging of the sample plates and the onion epidermal cells was realized,and the feasibility of the microscopic system was verified.The resolution of the resolution plate shows that the system has a lateral resolution of 512 lp / mm.The experimental results show that the system has the characteristics of three-dimensional structure of microscopic objects.(3)Finally,the basic theory and basis of compression sensing are introduced,and several algorithms are compared and the Lena image is reconstructed.At the same time,the compression sensing algorithm is applied to the non-coherent digital holography,the ideal object is numerically simulated,the hologram of the ideal object is obtained,and the reconstruction image and the compression of the single coaxial hologram are reconstructed by Fresnel diffraction algorithm The reconstruction algorithm of the reconstructed single coaxial hologram is compared.