Research Of A Cell Culture Control System Based On Microfluidic Chip

Cell culture technology is the most important technology in the fields of biology,medicine,and drug research.At present,the culture dishes,culture plates,and culture flasks are commonly used for cell culture work both at home and abroad,and the actual state of the extracellular microenvironment in vivo cannot be reproduced.Microfluidic chip flow channels are micron-sized and not only accurately control the extracellular microenvironment in time and space,but also quickly and accurately perform reproducibility tests,so it provides an important platform for the new generation of cell research.However,the existing microfluidic chip cell culture process still has the problems of uneven cell growth,and the control method of the culture medium perfusion is single,and it is unable to realize the automatic culture of different inoculation density at different growth rates.Uneven cell growth and different inoculation density and different growth rates affect the accuracy of subsequent experiments such as cell growth,cell viability detection,and drug induced apoptosis.In order to solve the defects of the above-mentioned microfluidic chip cell culture technology,the microfluidic chip cell culture control system was designed to achieve uniform cell growth and the automatic culture of different inoculation density at different growth rates.This paper is mainly about the design of microfluidic chip with culture medium flow uniform distribution and the culture medium perfusion predictive control method.The main content can be summarized as the following aspects:(1)Aiming at the problem of uneven cell growth,a microfluidic chip with culture medium flow uniform distribution was designed.Using the COMSOL simulation software and particle velocity experiments,the flow rate distribution in culture chamber of this microfluidic chip and the single-channel microfluidic chip was compared.(2)In order to achieve the automatic cell culture at different growth rates under different cell inoculation densities,a culture medium perfusion perdictive control method based on LS-SVM was proposed.The cell inoculation density and the three generations time of cell proliferation were selected as input,and the interval time of culture medium perfusion was selected as output.(3)The experimental platform of microfluidic chip cell culture control system was constructed,which consists of the field control device and the host computer control system,and the hardware and software design was completed.(4)After the experimental platform was constructed,the microfluidic chip cell culture experiment was conducted to analyze the relationship between the three generations time of cell proliferation and the interval time,and the relationship between the cell inoculation density and the interval time.According to the experiment,a large number of experimental data was obtained.According to the experimental data,a culture medium perfusion predictive control model based on LS-SVM was established.(5)The gastric cancer cells were cultured using the microfluidic chip cell culture control system and compared with that of a culture dish.The results of experiments indicate that the flow rate distribution in culture chamber of this microfluidic chip is more uniform than that of the single-channel microfluidic chip.Themicrofluidic chip cell culture control system can automatically control cell growth according to different cell inoculation densities and different three generations times of cell proliferation to increase the automation degree of cell culture,and more uniform cell growth than in the culture dish.This microfluidic chip cell culture control system cultured cells are more uniform than the cells cultured in the culture dish.