Research On Lactic Fermentation Hericium Erinaceus Polysaccharide Anointed Process

This thesis researches on the material of the dehydrated Hericium erinaceus entity for the test,with the ultrasonic extraction technology extracted Hericium erinaceus crude polysaccharide, and add to skim milk for malolactic fermentation.use the method of phenol-sulfuric acidmeasurement polysaccharides's change rule during the fermentation.when it was finished,the products need modulation through the add quantitative composite glue, the development of functional product is not only has Hericium erinaceus's unique flavor,but also polysaccharide.the test choice of the single factor experiment respectively for the ratio of liquid material, ultrasonic time, ultrasonic temperature three parameters.Through the single factor experiment, choose the best parameters for the response surface optimization experiment. The single factor concrete parameters settings are: the ratio of liquid material are15/1,20/1,25/1; Ultrasonic time are15min,20min,25min; Ultrasound temperature are35?,40?,45?. The response surface software analysis obtains the best extraction parameter of the ratio of liquid material is21/1, ultrasonic temperature is40?, ultrasonic time is20min. When using the optimized parameters of ultrasonic extraction, the rate of Hericium erinaceus crude polysaccharide is as high as4.852%. Without the response surface analysis software optimization of the parameters obtained Hericium erinaceus crude polysaccharide, the rate was4.434%, improve8.62%. From then on visible, the optimized parameters extraction can effectively increase the rate of crude polysaccharide, and convenient operation, the solvent consumption is less.The test for a single factor experiment and orthogonal test with the Hericium erinaceus extract, the quantity of lactic acid bacteria, adding amount of emulsion, adding amount of sugar, Base on sensory properties as evaluation indexes, the concluded of fermentation process that the best adding amount of ratio for Hericium erinaceus extract is25%, the quantity of inoculated is3.5%(Bulgaria lactobacillus: streptococcus thermophilus cultured=1:1)), the quantity of emulsion is65%, the quantity of sucrose is6%,Fermentation temperature40?, Fermentation tim,4h, Can achieve good taste and organization statoLactobacillus fermentation was finished, in70?, under25Mpa homogeneous. Addition a certain amount of glue made the sticky creamy.The test selection compound of carrageenan, agar, gelatin base on sensory evaluation index for orthogonal test. After the test, the conclusion of the best ratio for compound glue: the quantity of carrageenan is0.3%, the quantity of agar is0.4%, the quantity of gelatin is0.25%. In this parameter condition, can make the Hericium erinaceus fermentation anointed product viscosity is bigger, full-bodied of mouth-feel, also has better organization state and palatability. The test results show that in the fermentation process of lactic acid bacteria priority use lactose and glucose of milk powder, Hericium erinaceus polysaccharide was larger reserves. According to the mensuration, in the fermentation before and after has no great change, After fermentation Hericium erinaceus polysaccharide crude polysaccharide content is4.796%. Using this feature of the preparation of Hericium erinaceus polysaccharide product, its polysaccharide content is stable, the quality is bette. It can extend the shelf life, solve the requirements for reach a certain lactic acid bacteria living bacterium number and preservation of the question of short period.And use of polysaccharide can improve the body immunity, anti-aging and so on, physiological functions and have a synergistic fermented milk, according to the process of the development Hericium erinaceus fermentation anointed provide the theory basis for more Hericium erinaceus polysaccharide product research and development,and to rich the market types of fermentation.