Synthesis Of Chlorophyll Chlorin Derivatives And Study Of Cu²⁺ Fluorescent Molecular Probs

Fluorimetry is widely used for metal ions detection,which has some advantages such as high sensibility,simple operation and low price.In this paper, we study the pheophorbide-a methyl ester and amide two chlorin P6 fluorescent probe of copper ions.Firstly,chlorophyll mastic is used as raw material for synthesizing methyl pheophorbide-a via degradation in 5% sulfuric acid methanol.Then methyl pheophorbide-a and 3-aminopropanol are mixtured to obtain Amido Chlorin P6. chemical structures were characterized by elemental analysis, 1H NMR and 13 C NMR spectra. In addition, the ultraviolet visible light absorption spectra and fluorescence emission spectra of methyl pheophorbide-a and amido chlorin P6 were determined in this paper. In 300~800nm, methyl pheophorbide-a appears five absorption peaks. The maximum absorption wavelength was 665.5nm. The absorbance was 0.119. Amido chlorin P6 appear six absorption peaks. The maximum absorption wavelength was 660.5nm.After excitation at 414 nm, methyl pheophorbide-a emited fluorescence in 673.5 nm. After excitation at 412 nm, amido chlorine P6 emited intense fluorescence in 668 nm. The maximum absorption peak blue shift, after Cu2+ complexed either methyl pheophorbide-a or amido chlorine P6. At the same time, the fluorescence were also quenched. They had high sensitivity and selectivity to Cu2+.The 1:1 binding ratio between both methyl pheophorbide-a and amido chlorine P6 and Cu2+ was investigated using Job's plot method. The detection limit was 0.5 ?mol/L and 0.0746 ?mol/L in turn. Methyl pheophorbide-a and amido chlorine P6 can detect the copper ion in aqueous solution.