Preparation And Morphology Control Of The AuRu Nanoparticles And Its Application In The Hydrogen Peroxide Sensor

Hydrogen peroxide?H₂O₂?is the raw material or intermediate products of many industrial processes;many highly selective peroxidase involved in the enzymatic reaction product,and is an important ingredient in the food,drugs,environmental analysis.Therefore,rapid and accurate detection of hydrogen peroxide has a very important significance.At present,the methods of detecting the H₂O₂ are titration method,chemiluminescence,chromatography,spectrophotometry and electrochemical method et al,in which electrochemical method has been studied extensively because of its method is simple,high sensitivity and good linear range,fast and stable response signal.In this thesis,synthesis of a series of gold ruthenium alloy nanoparticles and to build the alloy nanoparticles sensor electrode to investigate the effect of the morphology and composition of the alloy nanoparticles on hydrogen peroxide detection performance.This thesis research work include:1?The spherical AuRu alloy nanoparticles?AuRu-NPs?was synthesized by water / AOT /cyclohexane reverse micelles system at room temperature,the average particle size is 4 ± 1nm,with a face-centered cubic structure.We build a hydrogen peroxide biosensor?HRP / AuRu-NPs were / GCE?modified by AuRu alloy nanoparticles and characteristic the biosensor with the electrode AC impedance;CV and i-t method are used to investigate the performance of the electrode detecting H₂O₂.The results showed that: in the experimental range,the results showed that there was linear relationship?R2 =0.9959?between response current and the concentration of the hydrogen peroxide.The detection limit is 5.02 × 10^-8mol / L.2?Agglomerates of AuRu nanoparticles was synthesized by N,N-dimethyl formamide system under 50? constant temperature,the average particle size is about 30 nm.It was used for modifying electrode of H₂O₂ sensor and constructing HRP / AuRu / GCE electrode.The characterization of the biosensor using the electrode AC impedance;CV and i-t method are used to investigate the performance of the electrode detecting H₂O₂.The results showed that: in the experimental range,the results showed that there was linear relationship?R2=0.9964?between response current and the concentration of the hydrogen peroxide.The detection limit was1.48?10-9mol / L.3?The stones like AuPtRu alloy nanoparticles?AuPtRu?was synthesized by water / AOT /cyclohexane reverse micelles system and the KBH4 as a reducing agent at room temperature,the average particle size is 6 ± 1nm,with a face-centered cubic structure.We consist a hydrogen peroxide biosensor?HRP / AuPtRu / GCE?modified by AuPtRu alloy nanoparticles.Thecharacterization of the biosensor using the electrode AC impedance;CV and i-t method are used to investigate the performance of the electrode detecting H₂O₂.The results showed that: in the experimental range,the results showed that there was linear relationship?R2=0.997?between response current and the concentration of the hydrogen peroxide.The detection limit was2.49×10^-8 mol / L.4?The spherical AuPtRu alloy nanoparticles?AuPtRu-NPs?was synthesized by water /AOT / cyclohexane reverse micelles system and the hydrazine hydrate as a reducing agent at room temperature,the average particle size is 4 ± 1nm,with a face-centered cubic structure.We consist a hydrogen peroxide biosensor?HRP / AuPtRu-NPs / GCE?modified by AuPtRu alloy nanoparticles.The characterization of the biosensor using the electrode AC impedance;CV and i-t method are used to investigate the performance of the electrode detecting H₂O₂.The results showed that: in the experimental range,the results showed that there was linear relationship?R2=0.9982?between response current and the concentration of the hydrogen peroxide.The detection limit was 1.75?10^-8 mol / L.