Preparation And Performance Research Of Collagen/Chitosan Composite Microspheres And Membrane

The aim of extracting collagen from tropical aquatic products residue and use it as raw material to prepare drug sustained-release microspheres and membrane, so as to turning waste into treasure and increasing added value at the same time. The concrete research content are as follows:(1)To optimize collagen extraction from fish skin by orthogonal test and BP neural network model. Methodps:First using the analytic hierarchy process (AHP) to determine the pretreatment of hainan tilapia skin. Using enzymatic extraction method and collagen extraction rate as evaluation index. The orthogonal test were based on four factors:NaCl concentration, concentration of acetic acid, extraction time and solid-liquid ratio, with which to screen out the best extraction process. With the data of orthogonal test as back propagation neural network input to optimize the simulating factors. The optimized process conditions were NaCl of 2 mol/L, concentration of acetic acid of 0.2 mol/L, extracting time of 21h and solid-liquid ratio of 1:20(m/v). The relative error of network prediction and actual measured values was 5%. BP neural network combined with orthogonal experiment can analysis the changing raw of factors that affecting enzymes method and find the best parameters without increasing test number.(2)Preparation of collagen/chitosan/nano-silicon composite microspheres by emulsion crosslinking method. With span80 and liquid paraffin as oil phase, introducing magnesium stearate as auxiliary emulsifier. Using SEM, FT-IR, UV, laser granularity instrument as characterization methods to study the influences of oil-water proportion?stirring speed? crosslinking agent and nano-silicon dioxide on microsphere forming and drug loading performance. The results showed that MS emulsification effect was good, and when nano-silicon dioxide concentration was 2%, Berberine hydrochloride microsphere: drug-loading rate was 15.66% and encapsulation rate was 66.52%. Vitamin D microsphere: drug-loading rate was 9.08% and encapsulation rate was 48.54%. the sustained release microshperes' particle size distribution was concentrated, Nano silicon dioxide make microsphere has a better sustained release performance, making the microspheres harder to decompose in low pH and reducing adhesion.(3)Using the hydroxyl on collagen and chitosan to interact with sodium trimetaphosphate to prepare composite membrane. By analyzing the structure, water imbibition, enzyme degradation performance and certain mechanical performance of different proportion of collagen/chitosan membrane with the fourier infrared spectrum (FT-IR), scanning electron microscope (SEM) as characterization, experimental results showed that non-toxic sodium trimetaphosphate's crosslinking effect was significant, for it not only improves the strength of membrane, and effectively reduces its degradation rate under lysozyme. When the collagen/chitosan quality ratio was of 2:3, porous structure appeared. By loading Hainan li medicine callicarpa nudiflora to improve the antiphlogistic and hemostatic effect of the membrane.In this paper, the research showed that the collagen can be extracted from aquatic products residue, which has great application prospect in the field of biomedical materials. Successifully using collagen as raw material to prepare drug sustained releasing composite microspheres and membrane.