Purification And Application Of Flavonoids In Honeysuckle Leaves

In order to improve the planting benefits and better utilization of honeysuckle, it is necessary to broaden the research scope of its active ingredient and strengthen the further study of health function. As the by-product of honeysuckle, honeysuckle leaves has been regarded as waste material and not been used for a long time, its output is about tenfold that of honeysuckle. Besides, the content of flavonoids is 2.78 times than that in the flowers, the antioxidant activity of rough flavonoids is higher than chlorogenic acid. In this paper, flavonoids in honeysuckle leaves was used to study its purification, the production process of health capsule. In addition, the toxicology assessment and antioxidant properties of capsule was also researched. The main results were as follows:(1) The optimal adsorption conditions were: 3.831 mg/mL of flavones in feed solution, 2.0 mL/min of flow rate, the best adsorption time was 3 h and loading volume no less than 100 mL. The elution conditions were as follows: 70 % ethanol with flow rate of 1.0 mL/min. After the D101 treatment, the concomitant were almost completely removed. Meanwhile, the purity of flavones increased from 7.14 % to 64.24 %. After 3 cycles, the resin should be regenerate.(2) Blended the flavonoid powder with(magnesium stearte+microcrystalline cellulose) according to the ratio for 5:1, 5 % PVP 95 % ethanol solution as the bonding agent for granulation. After granulating, the angle of repose was 25.92°,bulk density was 0.633 g/mL, which choose 0# capsule shells used in the production. During the process of production, environment relative humidity should be controlled below 66.89 %.(3) After a month at room temperature retention and 3 months accelerated test, there were no obvious changes among various examing indexes.(4) The tolerance to this flavonoid extracts was measured to be greater than 10 g/kg·BW, which was belong to the non-toxic level. Besides, the results of Ames test, mouse bone marrow cells micronucles test and mouse sperm malformation test ware negative, which indicated that the capsules has no mutagenic effect. 30 days feeding test was used in this study to assessment the safety. Compared with control group, the organ coefficients and biochemical indexes of the rats in each dose were no significant differences(p>0.05). The pathologic examination of main organs was the same, and no special pathological changes were found.(5) Using D-galactose induced aged mice as in vivo model, compared with this model aging control group, all dose groups were significantly lower in the levels of serum malondialdehyde(MDA) content for the experiment of antioxidant effects in mice, superoxide dismutase(SOD), glutathione peroxidase(GSH-PX) and total antioxidant capacity(T-AOC) were significant higher than the control group(p<0.05).