| Perilla frutescens seed is the desiccative ripe fruit of the lamiaceae annual herb plants of Perilla frutescens(L.) Britt., is a kind of traditional Chinese medicine commonly used in clinical. Perilla frutescens seed resources are widely distributed, it can be seen all over the country. The oil content of perilla frutescens seed is rich, it has a large amount of ?-linolenic acid. In view of the perilla frutescens seed oil(PFSO) are a good source of ?- linolenic acid, We should make full development and utilization of perilla frutescens seed resources. So the research was investigated the optimization of extraction process of perilla frutescens seed oil, the fatty acid composition, antioxidant activity, the inhibitory activity on xanthine oxidase, and the release in vitro of perilla frutescens oil microemulsion and so on. The main content and results were as follows:Perilla frutescens seed oil was extracted by cold and hot pressing method. On the basis of single factor of the moisture content, rotate speed and squeeze head temperature was chosen as the independent variable, the seed oil extraction rate as the response value, the cold pressed method preparation process of perilla frutescens seed oil was optimized by the Box-Behnken central composite design of response surface methodology. The results showed that the optimal preparation conditions were obtained as follows: moisture content 2.5% ? rotate speed 25 rpm ? squeeze head temperature 60?. Under the conditions, the yield of perilla frutescens seed oil was reached 34.28%, it's closed to predicted value 34.09%.The fatty acid composition of cold/hot pressed perilla frutescens seed oil was analyzed by GC-MS. The results showed that cold pressed PFSO was contains?-linolenic acid, oleic acid, linoleic acid, n-hexadecanoic acid and pentadecanoic acid.In hot pressed PFSO that ?-linolenic acid, linoleic acid and n-hexadecanoic acid was checked out, the oleic acid did not dectect, but there were some alcohols andalkanes for the reason that hot-pressing PFSO was through the high temperature steaming and frying and lead to oxidation and disintegrate of fatty acid.The order of the influence of different antioxidant on the oxidation stability of PFSO was: TBHQ > BHT > VE, added the TBHQ the shelf life of cold pressed PFSO was increased from 176 days to 480 days, the shelf life of hot pressed PFSO was increased from 112 days to 528 days. The antioxidant effect of combined antioxidant were better than single antioxidant, both of cold pressed and hot pressed PFSO the sorting to antioxidant effect was: 0.01% TBHQ+0.01% BHT > 0.01% TBHQ +0.01% VE > 0.01% BHT + 0.01% VE. Added the combined antioxidant of TBHQ and BHT the shelf life of cold pressed PFSO was increased from 176 days to 544 days,the shelf life of hot pressed PFSO was increased from 112 days to 560 days. The antioxidant effect of added the antioxidant and synergist 0.015% TBHQ+0.005% CA in both of the cold pressed and the hot pressed PFSO were the best, the shelf life of cold pressed PFSO was increased from 176 days to 528 days, the shelf life of hot pressed PFSO was increased from 112 days to 544 days.The result of ?- linolenic acid oxidation kinetics indicated that the ?- linolenic acid oxidation rule in cold pressed PFSO was more closed to index change rule(R2=0.908), conformed to the first-order reaction kinetics equation. And the ?-linolenic acid oxidation rule in hot pressed PFSO was more closed to linear variation rule(R2=0.930), conformed to the zero-order reaction kinetics equation.Cold/hot pressed PFSO were showed strong antioxidant activity, the sorts of scavenging effect of cold pressed PFSO to three kinds of free radicals was ·OH >O2-· > DPPH·, and the sorts of scavenging effect of hot pressed PFSO to three kinds of free radicals was DPPH· > O2- · > ·OH.The inhibition and enzyme kinetics of cold/hot pressed perilla frutescents seed oil on xanthine oxidase were investigated. The study demonstrated that the inhibition rates increased with increasing of the concentration of cold/hot pressed perilla frutescents seed oil from 1mg/m L to 6mg/m L, and the inhibition rates of cold-processed perilla frutescens seed oil showed a stronger inhibition rate. Also the study showed that the inhibition type of cold-processed and hot-processed perilla frutescens seed oil on xanthine oxidase both belong to the competitive inhibition. TheIC50 value of cold/hot pressed perilla frutescents seed oil were 7.07mg/m L,10.56mg/m L,and inhibitory constant Ki were 2.86mg/m L, 4.23mg/m L.The positive stage diffusion dialysis method was chosen to investigated the release of PFSO microemulsion in artificial gastric juice and p H 7.4 artificial intestinal fluid, then the situations of release were fitted respectively. The results showed that the release conditions of the cold/hot pressed PFSO in artificial gastric juice and p H 7.4 artificial intestinal fluid both accorded with Retger-peppas equation.In artificial gastric juice the cold/hot pressed PFSO microemulsion release equation were Q=12.37324t-0.38437,Q=5.69875t-1.15531 respectively, and in p H 7.4 artificial intestinal fluid p H 7.4 artificial intestinal fluid the cold/hot pressed PFSO microemulsion release equation were Q=1.03672t0.39456, Q=4.61072t0.16096 respectively. |
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