Synthesis And Properties Of Novel Metal Ions Fluorescence Probes Based On Rhodamine B

Because of the advantages of being very sensitive, “on-off” switch ability, rapid, safe and the features of sensing ions and neutral analytes, fluorescent probes has been used in of variety widea fields. In a number of the fluorescent probe, rhodamine is widely used to detect metal ions own to good light stability in a wide wavelength range, high fluorescence quantum yield, excellent rigid planar structure,low toxicity and so on. Therefore,the work was mainly carried out in the following aspects:First, we present a detailed introduction about the characteristics and structure of the fluorescence probes, And the status of research in recent years rhodamine fluorescence probes to do a systematic elaborate.Then, A novel turn-on fluorescent probe?BR? based on bis-?rhodamine? was synthesized, which recognized Fe³⁺ with high sensitivity and selectivity over other commonly coexistent metal ions in 7,:3=?v/v OTHF/H7.0?=p H 2 solution. Upon treatment with Fe³⁺, the chemosensor exhibited a significantly enhanced fluorescence and a visible color change for ‘naked-eye'. The sensing behavior between BR and Fe³⁺ was studied by Job's titration, which showed a 1:2 complex formation. In addition, the probe responded to Fe³⁺ with a linear range of 200- ?M and a low detection limit of M108.1-8?. Moreover, BR has been employed for imaging Fe³⁺ ion in living cells and for the determination of Fe³⁺ levels in human blood serum.Finally, A novel turn-on fluorescent probe?HL? based on Schiff base was synthesized, which recognized Hg²⁺ with high sensitivity and selectivity over other commonly coexistent metal ions in 7,:3=?v/v OOH/HCHCH7.0?p H223?28? solution. Upon treatment with Hg²⁺, the chemosensor exhibited a significantly enhanced fluorescence. The sensing behavior between HL and Hg²⁺ was studied by Job's plot, which showed a 1:1 complex formation. In addition, the probe responded to Hg²⁺ with a linear range of 100- ?M and a low detection limit of 3.7× 10-6 M. Moreover, HL has been employed for the determination of Hg²⁺ levels in the water samples.