Study On The Raman Immunosensor Based On A Composite Of Mesoporous Titanium Dioxide And The Study Of Photoelectrochemical Properties On The Mesoporous Tin Dioxode Film

Raman spectra was an important means of detection with less samples, faster detection speed, also the operation was very convenient, and it was easier to be identified. Raman spectra could be used for qualitative or quantitative analysis of no damage. It was mainly used in the detection of biological molecules, catalyst stru cture analysis and etc. In summary, the high sensitivity of raman spectra and specificity of the biochemical analysis make the raman immunosensor in this study may provide an interesting alternative tool for detection protein in clinical laboratory.In this work, a new type of Raman immunosensor was developed for the quantitative determination of ?-fetoprotein?AFP? based on a composite of the mesporous TiO₂ and silver nanoparticles. Transmission electron microscopy?TEM?, scanning electron microscopy?SEM?, XRD diffraction, UV-Vis, Raman spectroscopy were used to characterize the material. The mesporous TiO₂ and silver nanoparticles could enforce the Raman response of the sulphur benzoic acid. The composite material-labeled AFP antibody was applied to a sandwich-type Raman immunoassay for the detection of AFP. The immunoassay showed the linear range for AFP concentration from 0.01 ng/mL-0.6 ng/mL, and the detection limit of 0.005 ng/mL was obtained.This experience developed a gold and silver core-shell structure to enhance the Raman signal of mesporous TiO₂, so it could be used to detect the contect of AFP. Transmission electron microscopy?TEM?, scanning electron microscopy?SEM?, XRD diffraction, UV-Vis, Raman spectroscopy were used to characterize the material. The mesporous TiO₂ and gold and silver core-shell structure could enforce the Raman response of the sulphur benzoic acid. The composite material-labeled AFP antibody was applied to a sandwich-type Raman immunoassay for the detection of AFP. The immunoassay showed the linear range for AFP concentration from 0.001 ng/mL-0.4 ng/mL, and the detection limit of 0.0005 ng/mL was obtained.In this work, mesporous SnO₂ material was prepared through sol-gel method. The mesporous SnO₂ was characterized by transmission electron microscope?TEM?, scanning electron microscope?SEM? and X-ray Diffractometer?XRD?. The photoelectrochemical behavior of ITO/SnO₂ was studied, and the effect of potassium oxalate,triethanolamine and CdS quantum dots on the ITO/SnO₂. It was found that triethanolamine and Cd S quantum dots could enhance the photocurrent signal of ITO/SnO₂. The electron donor of triethanolamine for ITO/SnO₂ could strongly ameliorate the photoelectric capability of ITO/SnO₂. The photocurrent signal of ITO/SnO₂ was about 1.1 A. The CdS quantum dots on the ITO/Sn O₂ could strongly ameliorate the photoelectric capability of ITO/SnO₂. The photocurrent signal of ITO/SnO₂/CdS was highest than others, the photocurrent signal was about 1.4 A.