Assessment Of The Endocrine Disrupting Activity Of Industrial Water Bodies Based On Passive Sampling Technique

With the development of industry, waste water discharged from typical industrial zones containis different types of organic pollutants. These ingredients can interact in a nature environment and change the speciation or structures. They also have a potential risks to the ecological environment and human health. Currently single chemical analysis methods are difficult to assess their overall biological effects. In order to protect the environment and the ecosystem, there is an urgent need for evaluation and management of biological toxic effects of discharge to water bodies of the industrial zone. For the full supervision of the presence of toxic activity in industrial water body, this thesis mainly focused in the development of passive sampling technique and evaluation of endocrine disrupting activity based on recombinant yeast.The conventional sampling methods were not effective in collecting pollution information at low contaminant concentrations and could not be applied with significant fluctuation of pollutions. In this thesis polar integrated passive samplers(POCIS) was developed based on time weighted concentration of the water. When the temperature changes from 4 ? to 25 ?, the sampled rates of nonylphenol, atrazine, tetrabromobisphenol A(TBBPA) are increased by 37.8%, 27.7%, and 28.9%. The sampled rates of atrazine increased 1.53 times with the increase of salinity. The salinity has less impact on the sampling rates of nonylphenol and TBBPA. Under acidic conditions and basic conditions, the atrazine's sampling rate is lower 19.3% and 33.7% than the neutral conditions respectively. By increasing the agitation of water, the sampling rate of atrazine, tetrabromobisphenol A and nonylphenol increased by 2.35, 1.83 and 1.76 respectively.Because the presence of contaminants for endocrine cost much, this paper optimized the method of yeast estrogenic screen(YES). Arxula Adeninivorans can used maltose as carbon source, yeast secreted out of more phytic enzyme. When the yeast initial bacterial concentration is OD630 nm equaling to 2, the test results are more stable. When the test liquid yeast with a contact time of 16 to 24 h, phytase secretion of estrogen is positively correlated better. The best time for the enzyme reaction is(70 ± 5) min. The EC50 of estradiol is 83.80 ng/L. The EC50 of nonylpheno is 624.83 ?g/L. The EC50 of bisphenol A is 327.68 ?g/L.When the method of combining passive sampling and yeast estrogenic screen is applied to typical industrial zone drainage, the POCIS's sampling rate of endocrine disruptors need to be checked. Temperature changes from 4 ? to 35 ?, the sampling rate of 17 ?- estradiol increased 37%. High agitation speed can increased sampling rate 4 times comparing low stirring rate. Salinity and p H have little effect on the sampling rate. Finally, the sampling rate of endocrine disrupting chemicals is 0.25 L/d on the spot. The method is applied to Longgang River screening endocrine disrupting. There are many typical industries on Longgang River. Longgang River found estrogenic activity level equivalent to 3.1 ng/L E2. The results showed that passive sampling techniques can be recombinant YES to evaluation the biological effects of endocrine disrupting properties.