Study On The Physical And Chemical Properties Of Two Kinds Of Fructo-oligosaccharides And Their Effects On Probiotics

At present,according to the differences of the raw materials and the producing process,fructo-oligosaccharides could be divided into two kinds:FOSinulin and FOSsucrose.This study measured the water activity,Maillard reaction and thermal stability of two kinds of fructo-oligosaccharides to compare the differences of the physicochemical properties of FOSinulin and FOSsucrose.The results are as follows:(1)The water activity of FOSinulin is 0.1625,and the water activity of FOSsucrose is 0.1738;(2)Both two kinds of fructo-oligosaccharide products could have mild Maillard reaction;(3)The thermal denaturation temperature of FOSinulin is 64.3?,and the thermal denaturation temperature of FOSsucrose is 60.5?.Some studies have indicated that two kinds of fructo-oligosaccharides could regulate the intestinal flora,and promote the proliferation of probiotics in the intestinal tract.The effect of two kinds of fructo-oligosaccharides on the proliferation of probiotics was evaluated in vitro and in vivo experiments.The growth curve of probiotics with FOSinulin and FOSsucrose as carbon source in the culture medium respectively were got to see if two kinds of fructo-oligosaccharides had the same proliferation in vitro on probiotics in vitro experiment.In vivo experiment:120 male mice in Kunming were divided into 10 groups,12 rats in each group,and we would feed these mice the different doses of probiotic bacteria powder(two kinds of bifidobacterium bacteria powder)and fructo-oligosaccharides;The intestinal contents would be got,and the traditional culture method,PCR and real-time fluorescent quantitative PCR were used to study the proliferation of intestinal probiotics(Bifidobacterium and Lactobacillus).The results show:(1)Two kinds of fructo-oligosaccharides have no adverse effect on the normal growth and diet of the mice;(2)Both two kinds of fructo-oligosaccharides could promote the proliferation of bifidobacterium and lactobacillus;(3)Two kinds of bifidobacterium bacteria powder could promote the proliferation of bifidobacterium and lactobacillus;(4)From the growth curve in vitro,the FOSinulin has a better effect on two kinds of bifidobacteriums compared with the FOSsucrose in the overall;And from experiments in vivo,the FOSinulin has a better effect on the growth of bifidobacteria and lactobacillus compared with FOSsucrose.In this study,it turns out that the FOSinulin has a better effect on the growth of probiotics compared with FOSsucrose.