The Synthesis And Properties Study Of Rhodamine-based Functional Probes For The Control Of N-heterocycle

Since 1015,for the first book of the optics was published by Iraqi physicist,the application of light and light-based technologies became more and more mature.Fluorescence probe techniques,which use special fluorescence signal emitted by the probe molecule and achieve sensitive and selective detection towords the guest molecules.With the rapid development of optical instruments,the fluorescence probe techniques becoming more and more popular,not only in life science,environmental science and food science,but also in the field of information technology,material science and so on.The Rhodamine based fluorescence probes usually contain an easy-regulated structure of lactam ring,which is an ideal structure for design "OFF-ON" style fluorescence probes.At present,the modification of Rhodamine-based fluorescent probes mainly concentrate in the recognition group,which connected to the N atom of lactam ring?it always introduces some groups which contains N,O or S atoms?,Usually,the change of the identification group can not only have an effect on the recognition target,but also improve the qualities of the probe,such as sensitivity,selectivity,specificity,biological compatibility and reversible performance.This is an important breakthrough to design new probes based on xanthene structure.In this paper,a series of Rhodamine-based fluorescent probes,which controled by N-heterocycle,were designed and synthesized,their properties were also studied in detail.The contents of this paper as follows:?1?Introduction the basic composition and recognition principle of fluorescence probes and some common fluorophores.A review of N-heterocyclic regulated Rhodamine-based fluorescent probes and their application in the recognition of positive ions,small molecules,anions,biologically active oxygen and reactive nitrogen species and so on.?2?Based on the structure of Rhodamine B and 2,6-diaminopyridine?an important N-heterocycle compound?,a fluorescent probe RS1 was designed and synthesized with a unit of lactam ring structure,then by introducing a conjugated group and an aromatic structure,two novel fluorescent probe RS2 and RS3 were synthesized and its structure were characterized.The optical properties of those probes were studied,and then the three probes were applied to the lysosomal-targeted recognition in A549 cells successfully.?3?Based on the structure of Rhodamine B and coumarin-3-carboxylic acid,a novel fluorescent probe SH for detection of Hg²⁺ was designed and synthesized,and the structure was characterized.The optical properties of SH showed the probe was well sensitivity and selectivity,and a relatively wide pH range were allowed in the detection of Hg²⁺.