Authenticity Identification Method For Swellfun Wellbay Liquor

Liquor is one of special food with a long history in China,occupies an important position in the food industry.The liquor category and flavor types in China are diversity.At the same time,due to different wineries have imparity manufacturing technique and environment,the characteristic of their products are correspondingly different.In recent years,accompany with the rapidly development in liquor industry,many problems were exposed,such as the sham as the genuine and make and sell counterfeit goods in liquor market,etc.,which has a huge impact in the healthy development of liquor industry.Therefore,to establish a scientific and comprehensive liquor brand pattern identification method plays an important role in the healthy development of liquor industry.In this work,SwellFun Wellbay liquor as a main research object was analysised by GC-MS,ICP-AES and FT-IR to find out the best modeling parameters.Discriminant analysis was used to preliminarily establish liquor authenticity identification model for Swellfun Wellbay liquor.The main research contents are as follows.(1)The optimal analytical conditions of the GC-MS were determined:Chromatographic conditionAgilent CP WAX 52 CB capillary column(30 m ? 0.25 mm ? 0.25 microns);The initial oven temperature was 35 ?(4 min),which was ramped up at 5 ?/min to 110 ? and held there for 1 min.Then it was ramped up at 10 ?/min to 200 ? and held there for 20 min.99.999% pure helium was used as the carrier gas.The helium flow rate was 1.3 mL/min.The injection port temperature was 220 ? and operated in split mode(10:1),the sample size was 1 ?L.MS conditionThe mass detector was operated in the electronic impact(EI)mode at 70 eV and the source temperature was set at 230 ?.The quadrupole temperature was 150 ? and the interface temperature was 280 ? The detector was turn off when the qualitative time was between 3.3-4.0 min(Ethanol Peak).The mass spectra were scanned in the m/z 29-350 amu range.Liquor samples were analyzed by GC-MS in the optimal analytical condition.As the results,79 kinds of flavor substances in SwellFun Wellbay liquor,80 kinds in Wuliangye liquor and 84 kinds in Langjiu Liquor were identificated,including the esters,the organic acids,the alcohols,the ketones,the aldehydes,the phenols and the heterocyclic compounds,etc.The distribution characteristics of all kinds of flavor substance were compared between different brands of liquor.The result showed that,in Swellfun Wellbay and Wuliangye liquor,the relative content of esters compounds was highest,accounted for 64.7 % and 64.7 % of total flavor substances respectively.Nevertheless,it was comparatively low in Langjiu liquor,only accounted for 40.0 % of total flavor substances.But the content of the aldehydes and the alcohols were relatively high in Langjiu liqour,accounted for 26.6 % and 19.7 % of total flavor substances respectively,higher than that of Wuliangye and Swellfun Wellbay liqour.The ketones were accounted for 3.7 % of total flavor substances in Swellfun Wellbay liqour,higher than that of 1.7 % in Wuliangye liquor and 1.5 % in Langjiu liquor.The relative content of the acids in Swellfun Wellbay and Langjiu liquor was almost equal,accounted for 10.3 % and 10.4 % of total flavor substances respectively,but lower than that of 19.2 % in Wuliangye liquor.(2)The principal component analysis(PCA)was used for the GC-MS datas of liquor samples dimension-reduced processing,and 37,20 and 10 kinds of flavor substance were selected as modeling parameters,discriminant analysis was applied to establish liquor authenticity identification model for Swellfun Wellbay liquor respectively.Compared the effect of the three kinds of discriminant model of discriminant,as the result,the discriminant model with 20 kinds parameters was the best,the 37 kinds parameters model was the second and the 10 kinds parameters model was the worst.(3)The content of 16 kinds of trace elements in the liquor samples was determinated by ICP-AES and the distribution characteristics of these trace elements was research in different liquors.As the result,the content of Na,Ca,Mg and Al of Swellfun Wellbay liquor was relatively higher than that in Wuliangye liquor and Langjiu liqour.The content of K in Langjiu liquor was higher than that of Wuliangye liquor,but lower than that in Swellfun Wellbay liquor.Furthermore,Mg,Mn,Fe and Sr were not detectored in Wuliangye liquor,but only Sr in Langjiu liquor was not detectored.Furthermore,there were not significant distribution feature of Se,Zn,Ni,Cu,Cr and Cd in the three brands liquor.In addition,discriminant analysis was carried to establish authenticity identification model based on the 16 trace elements for the SwellFun Wellbay liquor.The initial discriminant rate of this model was 100 %,the discriminant rate of cross validation test was 95.6 %,and the correct identification rate of validation set samples was 89.4 %,which showed the 16 trace elements could reflect the characteristics of the different brands of liquor.The feasibility of liquor authenticity identification method based on trace elements was proved.(4)The infrared spectrogram of liquor samples were gathered by FT-IR and pretreated by a serial methods.Principal component analysis was used for IR datas dimension-reduced processing,and the principal components which cumulative variance contribution rates exceed 99 % were extracted as the modeling parameters.Combined with the discriminant analysis,the liquor authenticity identification model was established based on all wavelengths,fingerprint region and first derivative spectrum of liquors infrared spectroscopy respectively.The results showed that the discriminant effect of identification model which established by the wavelengths data of liquors infrared spectroscopy was higher,its initial discrimination rate was 95.6 %,and the cross validation test of discriminant rate was 91.7 %.Meanwhile,the correct identification rate of validation set samples was 89.4 %.