Preparation And Property Study Of Antimicrobial Materials Containing Poly(Hexamethylene Guanidine)

Bacterial infection has become one of the major threats to human health.Nowadays,antibiotics are widely used in the treatment of various bacterial infections,but the excessive use of antibiotics led to the emergence of drug-resistant bacteria and environmental pollution.Thus exploring safe antimicrobial materials to inhibit the growth of bacteria outside human body to reduce the harm to it is of great practical significance and wide application prospect.Poly(hexamethylene guanidine),with broad spectrum antimicrobial properties,low cost and non-toxicity,is an environmentally friendly disinfectant.In this paper,we prepared hydrogels and natural plant fibers with strong and long lasting antimicrobial activity via chemical modification based on poly(hexamethylene guanidine)hydrochloride,and study their physical and chemical properties in detail.Hydrogel,with inherent three dimensional structures formed by physical or chemical interactions,has been widely used in various biomedical applications including tissue engineering scaffolds,wound dressings and drug carriers.Antimicrobial hydrogels can avoid or reduce the use of antibiotics,having the potential for clinical application.In this work,we first synthesized polymerizable poly(hexamethylene guanidine)macromolecular precursor,and then prepared poly(hexamethylene guanidine)-polyacrylamide hydrogels under photo-initiated radical polymerization.The antimicrobial activity of the resultant hydrogels were characterized by Zone of inhibition and OD600 measurements.Since poly(hexamethylene guanidine)segments were covalently bonded to the skeletons of the hydrogels and could not be eluted after being washed for several times for a long time,the hydrogel exhibited good and long-lasting antimicrobial activity against S.aureus and E.coli.In addition,hemolysis and cytotoxicity assays demonstrated the excellent biocompatibility of the antimicrobial hydrogels,which is expected to become new biomedical materialsNatural plant fiber has been widely used as textile materials because of its abundant resources and relatively low cost.Besides,as natural plant fiber is easy to degrade,it has been regarded as natural renewable resources in line with the requirements of sustainable development.Fabrics based on textile materials are prone to bacterial infections in the environment and pose potential dangers to human when contacted with body.Therefore,it is meaningful to explore new textile materials with good antimicrobial activity via simple chemical modification.In this work,modified cellulose acetate fiber and cotton yarn based on poly(hexamethylene guanidine),taken as two examples of natural plant fiber,were facilely prepared by means of simple aqueous grafting modification.Zone of inhibition and OD600 measurements demonstrated the excellent strong and long lasting antimicrobial activity against both S.aureus and E.coli,making it a potential candidate as advanced textile materials.