Effect Of D-pinitol On Cholesterol Metabolism In Hyperlipidaemia Hamsters

The hyperlipidemic effect of D-pinitol was studied in hyperlipidemic hamsters during this investigation. A model of hyperlipidemic hamsters was established by feeding high-fat diet via male SPF hamsters. Then the hyperlipidemic hamsters were divided into four groups,including one model control group and three dosage groups[50,100,200mg/ (kg·BW d)], which were given a daily dose for 6 weeks respectively.In the whole treatment phase, some of the biochemical indexes including total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C)were tested and non-HDL-C was caculated to verify the effect of D-pinitol on hyperlipidemic hamsters. After 6 weeks treatment, all the hamsters were sacrificed and physiobiochemic and hemorheological indexes of CHE, T-AOC, SOD, CAT, GSH-X,MDA were tested. Folch metho method was used to test the levels of TC and TG in livers and faeces of experimental hamsters. The microscopical structure of the liver tissue and aorta were observed by HE staining. The fecal neutral sterol and acidic sterol were separated and tested by gas chromatography. RT-PCR was conducted to explore the effect of D-pinitol in regulating the mRNA expressions of key genes about cholesterol metabolism in liver and small intestine.The results suggested that after 6-week-treatment with D-pinitol, the levels of TC,TG, non-HDL-C, TC/HDL-C and AI in serum were significangtly decreased (p<0.05).The TC, TG levels in liver were significantly decreased (p<0.05), and the TC, TG-levels in faeces were significantly increased (p<0.05). D-pinitol significantly decreased the enzyme activities of CHE and the constration of MDA, significantly increased the T-AOC and the enzyme activities of SOD,CAT and GSH-X (p<0.05). The liver and arterial tissue slices of hamsters were observed by HE staining, and D-pinitol could improve the pathological symptoms effectively. Gas chromatography suggested that D-pinitol could significantly increase the fecal neutral sterol and acidic sterol (p<0.05)_RT-PCR results suggested that D-pinitol could down-regulate the mRNA expressions of HMGCR and ACAT2, significantly up-regulated mRNA expressions of LDL-R,SREBP2, CYP7A1,LXR?,PPAR? and ABCG5/CG8 (p<0.05).