Research On The Regulation Of Resistant Starch On Liver Injury Induced By Deep-fried Oil

A number of complex chemical and physical reactions take place during frying and it will damage the health once exceeded its limit. This research uses quantitative gavage to study the toxicity effects of deep-fried oil to the rat liver and the regulation of resistant starch. Wistar rats with a basal diet was divided into three experimental groups with different treatments to study the consumption of deep-fried oil to the glucose yields, lipid yields and hepatic functions and to reveal the functions of resistant starch to deep-fried oil induced injury, respectively. Meanwhile, we studied the relative expression levels of glucose and lipid metabolism related genes using qPCR. Finally, different expressed genes analysis and pathway significant analysis is researched using RNA high-throughput sequencing to reveal the mechanisms resistant starch intervention to the deep-fried oil induced disorder of glycolipid and liver injury in rats.Firstly, a large number of PAHs and polar compounds will produced during frying,such as total polar compounds was reached 32.14% (GB7102.1-2003 limit 27%) and PAHs reached 16.28 ?g/kg (GB2762-2012 limitlO ?g/kg). which was beyond the standards. The changes of body weight, food intake, hair color gloss, excretion and activity of rats in each group were monitored during the experimental time. After 4 weeks consumption of deep-fried oil, deceased food intake, weight loss, dim hair color and abnormal activity raised in the rats, but not occurred in the resistant starch intervened group.Secondly, we researched the glycolipid level and liver function biomarkers of rats in each group. The results showed that the consumption of deep-fried oil will induce liver injury resulting in high levels of aminotransferase (1.45 and 1.56 times to normal control group), pathological changes of liver sections and the disorder of glycolipid metabolism in rats.Thirdly, we studied the relative expression levels of related genes based on qPCR. The results showed that the consumption of deep-fried oil will decrease the expression levels of glycolipid metabolism related genes compared to the control group. Interestingly, resistant starch reflected good effects to regulate these gene expressions to normal levels, especially the genes of' Insig1, Insig2, Fads1 and ACOX1, which indicating that resistant starch really attenuated the side effect of deep-fried oil.Finally, the RNA-seq analysis showed that the hydrolase activity, catalytic activity and binding were dominant in molecular function after the consumption of deep-fried oil and resistant starch have a protective effect to the normal cellular structure.The results and conclusions in this thesis initially revealed the hepatotoxicity and the genotoxicity of deep-fried oil, especially the protective effect of resistant starch. These results not only provide reference for the people to understand the side effects of deep-fried oil to health, but also provide a feasible way for looking for functional factors against deep-fried oil induced injury. The results also provide some instructions for a healthy diet.