Construction And Application Of New Material Based On Fluorescence Recognition

New extraction material,preparation method and detection methods are developed constantly in the development process of solid-phase extraction.Fluorescence detection was used in the detection of trace materials with high sensitivity,low detection limit,high accuracy and convenient operation in numerous testing method.Three kinds of different material were constructed to detect analytes based on the fluorescent recognition in this thesis.Floxacin own fluorescence properties,so it can be used for quantitative determination with fluorescence detection;or qualitative and quantitative analysis can be carried out by the change of fluorescence intensity of materials after combining with analytes.1.Nickel foam was used as a solid-phase extraction support to prepare temperature sensitive molecular ly impr inted polymers using ofloxacin molecules as the template.Nickel foam was modified by self-polymer ization of dopamine and silane,surface polymerization was carried out with N-isopropyl acrylamide as a temperature sensitive monomer,methacrylic acid as functional monomer and ofloxacin as template molecule.The fluorescence intensity could be observed by the naked eye under ultraviolet light after adsorbing ofloxacin and fluorescence intensity increased with increasing concentration of adsorbed solution.Fluorescence intensities of molecularly imprinted polymers were different from non-molecularly impr inted polymers after adsorbing.A detailed evaluation was made by different characterization methods and various adsorption experiments.Finally,the molecularly imprinted polymer was used as an adsorbent in solid phase microextraction coupled with high performance liquid chromatography to detect ofloxacin,norfloxacin and enrofloxacin in Yellow River water.Recoveries was 93.2-108.3% and limits of quantification was 0.014-0.020 mg L-1.2.Two kinds of solid-phase extraction materials with polysulfone as coating based on nickel foam were prepared by dip-coating method.The materials that polysulfone as coating based on nickel foam can absorb and enrich floxacin drugs specially.The adsorption capacity of the material for floxacin will be increased by adding molecularly impr inted polymers of ofloxacin in the preparation process.The preparation method is simple and reproducibility is good,and the forming process of the material can be controlled well.Two kinds of materials have been verif ied by scanning electron microscope and various adsorption experiments.Finally,two kinds of materials were used to detect analytes of the actual water samples and biological samples.The recovery experiment was carried out and a better recovery rate was obtained.The recovery rate of water samples and biological samples were 90.0-104.8% and 79.3-107.1% respectively.The repeatability of the materials was found to be very good by intra and inter day experiments,and the RSD value of two kinds of polysulfone were 1.0-9.9% and 1.7-10.3%.The quantitative limit of three kinds of floxacin were 3.0-6.2 ?g L-1 by the combination of the material and the high performance liquid chromatography.3.Boron and nitrogen elements co-doped carbon dots were prepared by solid phase synthesis using the compounds containing the structure of phenylboronic acid.These uniform particles were characterized by various techniques and their fluorescence property was investigated carefully.Fluorescence intensity of these carbon dots can be changed after the combination of monosaccharide under pH 9.0 and then used to the quantitative determination of monosaccharide with the limit of quantitative analysis as low as 2.5 ?M for fructose.Different types of monosaccharide can be distinguished by combining use of the carbon dots from phenylboronic acid and 3-aminophenylboronic acid.