Cu_2-xSe Nanoparticles Involved Catalytic Chemiluminescence And Their Application In Biochemical Analysis

In recent years,various kinds of nanoparticles were applied in chemiluminescence systems because of their fantastic catalytic,biocompatible and self-assembly property.It was successfully used in biological analysis,making a major breakthrough such as improving sensitivity,shorting analysis time.This article reviews some progress in nanoparticles involved in chemiluminescence system.In the past time,the chemiluminescence system was confined largely tomolecular and ion system.With the development of nanotechnology,the liquid chemiluminescence systems invoved nanoparticles were noticed.It was founded semiconductor nanoparticles copper selenide could strongly enhanced the chemiluminescence signal as catalyst.According to the reported literature,the most widely used nanoparticles were noble metal nanoparticles,only few data was reported about copper selenide nanoparticles.Therefore,this paper applied copper selenide nanoparticles into liquid chemiluminescence system.We discuss the catalytic mechanism and use this system to detect biological molecular.The result showed the sensitivity was improved and the linear range was broadened.The concrete research content including the following three aspects:(1)Cu2-xSe nanoparticles were used as a catalyst to enhance the chemiluminesence signal,and applied this enhanced chemiluminescence detection method to determine cholesterol.In this technique,the nonstoichiometric Cu2-xSe nanoparticles can facilitateelectron-transfer processes and decompose H2O2 into OH· and O2-,which are commonly considered to be key intermediates in the enhancement of luminol chemiluminescence.Compared with other detection methods,this luminol chemiluminescence system catalyzed by Cu2-xSe nanoparticles was found to be more sensitive to the level of cholesterol.Besides,this catalyst is also recyclable.(2)We used negatively charged Cu2-xSe nanoparticles as catalyst in chemiluminescence system to establish a chemiluminescence “turn on-off” sensing system to label-free detect lysozyme.Cu2-xSe nanoparticles could strongly enhance chemiluminescence signal,once lysozyme was added into the system,Cu2-xSe nanoparticles aggregated and the chemiluminescence signal was sharply decreased.In comparison with previous methods,our approach takes advantages of label-free,simplicity,speediness and low cost.More importantly,the strong chemiluminescence signal caused by Cu2-xSe nanoparticles offered a high sensitivity.And what's more,it also could be applicable to the determination of lysozyme in urine samples.(3)The synthesis and application of functional composite chemiluminescence materials based on Au NPs have been developed rapidly in recent years.However,to date,multifunctional Au NPs-based materials with chemiluminescence activity have rarely been explored.Luminol-type chemiluminescence reagents such as luminol,isoluminol and N-(aminobutyl)-N-(ethylisoluminol)(ABEI)are good reductants,which have been successfully used to reduce HAuCl4 for the preparation of chemiluminescent functionalized gold nanomaterials.we report the first Au-based composite material covalently functionalized with luminol via a green and facile chemical approach It has good chemiluminescence activity.luminol existed on the surface of AuNPs by virtue of Au-N covalent interaction in the as-prepared nanocomposites.Cu2-xSe nanoflower exists via Au–N covalent bonds between Au nanoparticles and amino groups of luminol.It has great potentional to detect reactive oxygen species in the future.