| Chemiluminescence (CL) is defined as the emission of light from an electronically excited state species which is produced during the course of a chemical reaction, and is observed when an electronically excited product or intermediate formed during a chemical reaction decays to the ground state by emitting a photon. CL offers three important advantages for chemical analysis: (a), because of its inherent sensitivity, low detection limits are possible, often in the femto ~ attomole range; (b), for most analytes, the linear dynamic range is several orders of magnitude; (c), instrumentation is relatively simple, which aiiows techniques to be developed for on-site analysis when more expensive or cumbersome instrumentation might not be suitable. Nowadays CL analysis has been widely used applied in environmental science and life science and has become one of the most powerful techniques for use in analytical chemistry. This paper deals with the following six systems as examples to investigate the application of CL analysis in environmental fields and biochemical analysis. 1) Ce(IV)-RH6G-phentolamine system;2)K3[Fe(CN)6]-Juminol-phertolimine systems; 3) potassium hypermanganate-phentolamine system; 4) potassiumhypermanganate-luminol-dobutamine hydrochloride system; 5)plant tissue-based biosensor for the determination of oxalate.6) potassium hypermanganate-luminol-Hg( II) system.1. Ce(IV)-RH6G-PHENTOLAMINE SYSTEMA new chemiluminescence (CL) method is proposed for the determination of phentolamine, which is based on the reaction between studied drug and Ce(IV) in a nitric acid medium and measurement of the CL intensity produced by rhodamine 6G used as a sensitizer. In the optimum conditions, CL intensities are proportional to concentrations of the studied drug over file range 1 10-9 ~ 1 10-6 g/mL with a detection limit of 4.1 10-10 g/mL. The relative standard deviation (RSD) is 3.4% for l.0 10-7 g/mL phentolamine (n=11). The method has been applied to the determination of studied drug in injections and biological fluids with satisfactory results.2. POTASSIUM HYPERMANGANATE-PHENTOLAMINE SYSTEM;A fast and simple chemiluminescence(CL) method for the determination of phentotemine mesylate is proposed based on its direct oxidation with potassium permanganate in acidic media. In the optimum conditions, CL intensities are proportional to concentrations of the studied drug over the range 0.05 ~ 6 g/mL with a detection limit of 0.01 g/mL. The relative standard deviation (RSD) is 3.0% for 4 fig/mL phentolamine mesylate (n=11). The method has been applied to the determination of studied drug in injections and biological fluids with satisfactory results.3. K3[Fe(CN)6]-LUMINOL-PHENTOLAMINE SYSTEMS FOR THE DETERMINATION OF PHENTOLAMINEA novel flow injection chemiluminescence enhancement method for the determination of Phentolamine based on luminol-Fe(CN)63"-OH~ system is developed. The method is simple, rO f.apid and effective to determine Phentolamine in the range of 10" -10" g/mL with a detectio n limit of 3.OX 10"9g/mL.the relative standard deviation is 3.3% for the determination of 6.0 X10"7g/mL Phentolamine (n=ll). The method has been applied to determine the content of Phentolamine in urine and serum with satisfactory result .4. POTASSIUM HYPERMANGANATE-LUMINOL-DOBUTAMINE HYDROCHLORIDE SYSTEMA new chemiluminescence (CL) method, which based on enhancement effect of dobutami ne hydrochloride on the CL intensity produced by potassium permanganate oxidation on lu minol is proposed for the determination of dobutamine hydrochloride. Under optimized cond itions, the response of CL on concentration of dobutamine hydrochloride is linear in the ran ge 1.0xlO~3~0.5 jig/mL with a detection limit (3a) of S.OxlO"4 p.g/mL. Relative standard dev iation (RSD) is 3.39% for 0.4 ug/mL dobutamine hydrochloride (n=ll). The proposed raeth od has been successfully applied to the determination of dobutamine hydrochloride in pharm aceutical preparations, plasma...
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