Investigation And Application Of Novel CL Method Based On Hydrogen Peroxide As An Oxidant

Chemiluminescence?CL?is the electromagnetic radiation produced in a chemical reaction without added light and energy.In recent years,because of its advantages such as high sensitivity,wide linear range,simple instruments,CL technique is widely used in food,biology,medicine,chemical industry and other fields.In recent years,reactive oxygen species?ROS?and related compounds were paid more and more attention,and it has been already developed to a specialized research topic gradually.Therefore,ROS is investigated frequently.Among them,hydrogen peroxide?H₂O₂?is one of the most common and mature species.H₂O₂ was known as a green oxidant,and could oxide other luminescence reagent,resulting in CL signal.Low selectivity does be the main weakness of CL technique,but this weakness can be overcome by coupled with separative techniques.HPLC is the most frequently used one.On the other hand,owing to the weakness CL emission of the traditional CL system,people centered their interest on some new nanomaterial for the purpose of enhancement of the CL intensity.Au NCs have been made as promising candidates in the fields of CL and other fields due to their advantages such as good water solubility,easy synthesizing.Nowadays,investigation and application of CL system based on hydrogen peroxide as an oxidant developed step by step.Two parts are included in this thesis: The first part is a review,which briefly described the research progress of green oxidant hydrogen peroxide in CL.The second part is the research report.The works are included in the followings:1 Determination of PG in edible oil by luminol-H₂O₂ CL system coupled with high-performance liquid chromatographyBased on the inhibiting effect of propyl gallate?PG?on the chemiluminescence?CL?between luminol and H₂O₂ in alkaline solution,and the combination technique of high-performance liquid chromatography,a novel and selective post-column CL detection method is established.The analysis was performed using a C18 column with isocratic 60:40 methanol/water.Under the optimum conditions,the concentration of propyl gallate was linear from 9 × 10-6 mol L-1 to 1 × 10-4 mol L-1 with a detection limit of 2 × 10-7 mol L-1.The relative standard deviation?RSD?of the method was 3.2% with 11 repeated measurements of 1×10-5 mol L-1 PG.The method was successfully used for the determination of PG in edible oil.2 Au NCs-enhanced chemiluminescence from NaHSO3–H₂O₂ and its analytical applicationWater-soluble Au nanoclusters capped with bovine serum albummine?BSA?was synthesized.It was found that the ultra-weak chemiluminescence?CL?emission from the sodium bisulfite?NaHSO3?–H₂O₂ system could be enhanced by gold nanoclusters?Au NCs?.The as-prepared Au NCs were applied to the NaHSO3–H₂O₂ system for the first time,and a decreased CL was observed in the presence of trypsin.This novel CL system based on Au NCs–NaHSO3–H₂O₂ was developed for trypsin determination.Herein,UV-visible spectroscopy and fluorescence spectra coupled with radical scavengers were used to explore the possible mechanism.Finally,the proposed method was successfully utilized to detect trypsin in human urine samples with good accuracy and precision.3 Enhanced chemiluminescence of the Rhodamine 6G-H₂O₂ system by BSA-stabilized Au nanoclusterAu NCs capped with bovine serum albummine?BSA?has good biological compatibility and optical properties.It was found that the ultra-weak chemiluminescence?CL?emission from Rhodamine 6G-H₂O₂ system could be enhanced by the as-prepared Au NCs.Different factors such as concentration,flow rate were also studied,and CL spectroscopy and fluorescence spectra coupled with radical scavengers were used to explore the possible mechanism.