Development Of A Biodosimetry Based On Variation Of Iron Element Valence

In twenty-first century, the radiation accidents happen frequently with the rapid development of nuclear energy and nuclear technology applications, as well as the nuclear terrorist attacks become more complex. Biodosimetry plays an important role in estimating the absorbed dose of exposed persons. We need to develop more excellent biological dosimeter since there are many deficiencies in traditional ones. The aim of this paper is to further study serum iron biological dosimeter. At the same time, to study the methmoglobin changes of organism irradiated by 60 Co γ-rays and explore its feasibility as a biodosimetry.To further study the serum iron dosimeter we used the vitro serum of human peripheral blood as the experimental model. The concentration of serum iron in γ-rays irradiated serum was detected using ELISA. The male and female dose-response curves followed the linear-quadratic model were established, respectively. Time-response relationship demonstrated that the serum iron was stable within 2 d. With the dose-response linear-quadratic curve, the absorbed dose was close to the double-blind dose, and the 95% confidence interval of the estimated value was narrow.At first, we used the mouse as the experimental model in the study of methmoglobin as a index on biodosimetry from three aspects of dose-response、time-response and dose-reconstrution. Then, we used the vitro red blood cell of human peripheral blood to further study methmoglobin as a biodosimetry. The male and female dose-response curves followed the linear model were established, respectively. Time-response relationship demonstrated that the methmoglobin was stable within 3 d. With the dose-response linear curve, the absorbed dose was close to the double-blind dose, and the 95% confidence interval of the estimated value was narrow.The serum iron and methmoglobin dosimeters which based on ELISA are sensitive、fast、simple and inexpensive. They are suitable for use in a nuclear accident and radioactive accident of acute uniform dose assessment. Both of serum iron and methemoglobin have the potential as biological dose estimation method, which is important for the development of biological dosimeter.