A Driving Device For Several Reagents Based On Air Pump In Consecutive Reaction System

Microfluidic technology has developed rapidly in the 21st century,and the demand for high-precisicon microfluidic driving technology is gradually increasing.The pneumatic drive has a significant advantage in microdroplets,a branch of microfluidic.However,only three foreign companies sell the pneumatic drive system with high precision at present,and the development of domestic product is still in the early stages.Therefore,the project is aimed at a pneumatic driving device for several reagents with high precision and high sensitivity,to achieve stable injection and solve the problem of pulsation in the syringe.This project mainly studied a pneumatic drive system with two independent pressure outputs.Take advantage of modular design ideas during hardware and software design.The PID algorithm had been applied to ensure the efficiency of pressure regulation,while the closed-loop control had been used to ensure the accuracy.This system has a wide range of pressure control(10 mBar?10 Bar),short System response time(0.3 s)and high stability(±1 mBar).And the outputs of two independent pressure can be applied to microdroplets directly.In addition,the microdroplets experiment was carried out by means of the pneumatic drive system.Photos were collected during the experiment by digital microscope.And ImageJ,a software,was used to pretreat the droplets images.The droplets sizes were uniform and had good monodisperse.And there is a linear relationship between the water pressure and the droplet volume.By the way,a certain proportion of the water and oil pressure was needed to ensure the steady generation of droplets.Further,extended research had been carried out based on the work of the pneumatic drive system.A variety of waveform pressure generation were achieved,such as sine wave,square wave,triangular wave,sawtooth wave and other conventional waveform.The output frequency of square wave and pulse wave is less than 14 Hz,while the output frequency of sine wave,triangular wave,and sawtooth wave is less than 1 Hz,because of the limit of the actuators and sensors in the system.Besides,S070B-5DC-M3 was chosen to design the pneumatic micro-pump control system.Moreover,LVM09 and INKX0514750A were used to design electro-liquid system,to control the opening and closing of the liquid circuit.Additionally,the electro-gas system and electro-liquid system were combined together to control multi-channel fluid independently.Continuous flow,plug,droplet and other fluid control methods can be fulfilled fast and conveniently.And then the color reaction of acid and alkali was achieved using the driving device which is for several reagents' delivery.Last but not least,the system can be used in a variety of microfluidic chips,and its prospect is broad.