Research On Design And Analysis Of Digital PCR Tester

Digital PCR test technology is through the sample is divided into tens of thousands or even millions of micro-chamber for a separate reaction and amplification,and then by fluorescence detection method to determine the number of target genes,according to the target sample gene content,to determine The type of disease.Currently on the market more mature PCR tester for the fluorescence quantitative PCR tester,the use of technology compared to the traditional water bath PCR detection technology has been greatly improved,but its dependence on the fluorescence curve and Ct value,subject to greater environmental impact,Can not meet the medical industry’s high-precision requirements,and the application of digital PCR tester is a good solution to this problem,its detection does not depend on the standard sample and C_t value.The development of digital PCR tester for the trend,and the current digital PCR tester on the market by foreign companies monopoly,equipment,expensive,late maintenance inconvenience.Therefore,relying on scientific methods to develop with independent intellectual property rights,low cost digital PCR tester has important practical value.In this paper,the structure design of digital PCR tester is the focus of research.Through the combination of theoretical calculation and computer aided design,based on the rationality,appearance and convenient maintenance of the whole structure of the whole instrument,based on the latest generation PTC Creo 3.0 software,ADAMS and ANSYS software to complete the digital PCR mechanical structure design and performance analysis.This paper first introduces the composition of the digital PCR tester,studies the working principle and working flow of the digital PCR tester,and designs the appropriate scheme according to the function of the designed instrument.Designed to use a screw stepper motor and a linear sliding guide combination of digital PCR tester three-dimensional sampling platform drive system,through the design of double-layer injection needle,to achieve repeated sampling,to improve work efficiency.A microfluidic microfluidic digital PCR fluorescence detection method was designed to overcome the shortcomings of the number of droplets in the existing microtiter digitalPCR test system and the fluorescence compensation.Through the combination of theoretical calculation and practice to complete the digital PCR tester screw stepper motor and sliding guide selection,and select the required pumps,valves and various types of sensors to achieve its basic functions.At the same time,the design of key components.The digital PCR tester was designed for chassis design,box material selection and overall assembly and layout of the instrument.Based on ADAMS,the three-dimensional sampling platform of digital PCR detector was used to analyze the motion performance,and the finite element analysis of the sampling needle was carried out by ANSYS software.The virtual simulation results show that the design of the parts is reasonable and the design of the motor structure is satisfactory.Finally,the ability of digital PCR detector to identify the fluorescence signal is tested.The results show that the fluorescence signal can be well identified,the droplet interval is uniform,the fluorescence intensity is basically the same,the yin and yang segmentation is obvious.