| Avian gout, which is one of the common chicken diseases all over the world, has a relatively high morbidity and mortality.And high dietary calcium and kidney-type infectious bronchitis virus (IBV) is one of the leading causes of disease. There are many researches have been done in the pathogenesis area, but still lack clear details.1501-day-old Hesse Alex layers have been choosed as the experimental animals.they were randomly divided into control group, the high calcium group.and virus group and kept to31-days-old, the high calcium group (Fed with3.81%calcium diet), and virus group (According to the EC50of kidney-type IBV virus in chick, each chicken inoculate with an allantoic fluid0.2mL at a concentration of10"5/0.2ml). The control group and virus group chickens fed with complete diet (with0.875%calcium diet). To copy the cases of gout, clinical pathology and histopathology methods have been used to study the blood and serum ion of blood, liver function and other biochemical changes in the process of IBV-induced chicken gout of high calcium diet, such as fat within the liver mitochondriaquality over the change of the oxidation products and antioxidant enzyme systems and p53protein expression in the liver at17,24,1Iday. The Experimental results show that:1?Both high calcium diet and IBV could induce chicken gout, resulting in diarrhea, slowing the growth and ureteral urate deposition.2?Both high calcium diet and IBV could induce the composition change of chicken blood.Compared with the control group, at day17the blood test of high calcium group showed a significantly decrease in white blood cell count, erythrocyte count, hemoglobin content and hematocrit; at day24, the blood test showed a reduced erythrocyte count, hemoglobin content and hematocrit, while the white blood cell count had a trace to increase; at day31, the white blood cell count, red blood cell count, red blood cell and hematocrit are increasing and the hemoglobin content had a trace to increasing. In virus group, at day17, the white blood cell count had a trace to increase, while erythrocyte count, hemoglobin content and hematocrit are tend to decrease:at day23and31. all of the tested white blood cell count, erythrocyte count, hemoglobin content and hematocrit showed a significant increasing.3?Both high calcium diet and IBV can induce the electorlytes changes in serum.Compared with the control group, at day17and24, the calcium group showed significant decrease in serum potassium and phosphorus, while serum chloride, sodium and pH value showed significant increase. At day17serum calcium tended to increase.at day24significant decrease in serum calcium.At day31, the calcium group showed significant decrease in serum potassium and phosphorus, and serum calcium, sodium and pH value showed a trace to increase, while the serum chloride increase greatly. In virus group, at day17and24, serum potassium and sodium showed significant increase, while serum phosphorus and calcium had a trace to decrease, and serum chloride and pH value had a trace to increase. At day31. serum potassium showed significant decrease, serum sodium showed significant increase, serum phosphorus and calcium had a trace to decrease. This prove that the high calcium diet will induce chicken hypercalcemia, both high calcium diet and IBV will induce the chicken hypokalemia. hypernatremia disease, highblood chloride disease, hypophosphatemia, metabolic alkali poisoning.4?Both high calcium diet and IBV will affect the liver fuction.Compared with the control group, at day17, total protein, albumin, alanine aminotransferase tended to increase, direct bilirubin. and total bilirubin was significantly lower. At day24, total protein and albumin tended to increase, direct bilirubin on a downward trend; total bilirubin was significantly decreased, and alanine aminotransferase increased. At day31. total protein and albumin were significantly increased, direct bilirubin and total bilirubin is on a downward trend, Alanine aminotransferase tended to increase. In virus group, at day17. total protein and albumin tended to increase the direct bilirubin was significantly decreased and total bilirubin, alanine aminotransferase significantly increased. At day24. total protein, albumin, total bilirubin were significantly increased, there was a downward trend in direct bilirubin, alanine aminotransferase tended to increase. At day31. total protein and albumin tended to increase the direct bilirubin was significantly decreased, and elevated total bilirubin, alanine aminotransferase increased significantly.5?High calcium diet, IBV chicken liver mitochondrial antioxidant function.Compared with the control group, the high calcium group at day17of the test, MDA was significantly lower, SOD significantly increased, XOD has decreased; at day24, MDA was significantly increased, there was a downward trend of the T-AOC and SOD significantly decreased, XOD tended to increase. At day31, MDA was significantly increased, the activities of SOD, T-AOC on a downward trend, XOD tended to increase. In virus group, at day17, MDA was significantly increased, the activities of SOD, T-AOC on a downward trend, XOD tended to increase. At day24, MDA was significantly increased, the T-AOC increased significantly reduce virus, SOD, XOD of the virus group significantly increased. At day31, a downward trend in MDA, T-AOC were significantly increased, SOD significantly reduced, XOD significantly higher.6?High calcium diet-induced chicken gout will cause liver congestion; kidney-type IBV may be induced chicken gout can cause liver bile salt deposition.7?High calcium diet and IBV can enhance the expression of p53protein in the young hens kidney tissue. |
PDF Full Text Request