| Lily (Lilium spp.) is a Liliaceous (Liliaceae) Lily (Lilium) plant in general, is a perennial bulbous herbaceous plants. Because of its tall, large flowers, financial ornamental, medicinal, edible as one, at home and abroad are widely cultivated ornamental and economic plants. At present, There are about115(var, subspecies) in the whole world, mainly distributed in the Northern Hemisphere temperate and boreal regions. China is the world Lily's main distribution centers, had founded that there were55species and18varieties in China. In recent years people did much work and utilization of germplasm resources of Lilium at home and abroad, but studied on Changbai Mountain lily with its quantitative classification and ISSR molecular marker research has not been reported.Changbai Mountain is one of the concentrated distribution area of wild lily in our country, and there are9species2varieties in there. Due to the special geographical position and climate environment, so the lily varieties, and the same ecological types varied greatly, genetic diversity, but at present a lot of resources have not been utilized and serious destruction, diversity reduced gradually. To solve this problem, we investigated and collected the lily on the northeast of Changbai Mountain, using ISSR molecular markers and quantitative classification method on the72Lily samples of4Lilies,and studies about their genetic diversity research, with a view to explore the genetic variation for Lily, And also provides the basis for building the core germplasm resources of the Lily and the resources science preservation.The main results are as follows:1. By adjusting Taq DNA polymerase, dNTPs, primer and annealing temperature were optimized. The optimal ISSR-PCR reaction conditions for Lilium. were determined as follow, in20?L PCR reaction system,20ng/?L template DNA,0.5U Taq DNA polymerase,0.3mmol/L dNTPs,0.5?mol/L primer.9ISSR primers which are suitable for Lilium. were screened, the optimal annealing temperature for the ISSR reaction was52.2?54.3?.2.9primers selected from35primers were used for ISSR amplification of the materials of Lilium. A total of159bands were generated,of which158bands were polymorphic bands(the percentage of polymorphic band, PPB=99.37%).Every Primer could amplify scope of14-24, averagely amplify17.67bands, among which17.56bands were found to be polymorphic.3. Through to the72Lily materials on ISSR molecular markers,used the NTSYSpc-2.10e software to experimental data,72materials of Lilium. could be clustered into4groups and14subgroups. according to the dendrogram.four kinds of all the test materials were independently classified into one group, the group ? L.distichum were divided into7subgroups; the ? group of L.dauricum were divided into2subgroups; the group III L.leicbtlinii var.maximowiczii were divided into3subgroups; the IV group L.cernuum were divided into2subgroups.4.The SPSS software was used to analyze the genetic relationships of the Lilium. using32morphological characters to the cluster analysis.72materials of Lilium. were divided into3groups and8subgroups. the group1was divided into2subgroups, the subgroup la for L.leicbtlinii var.maximowiczii, the subgroup Ib for L.dauricum; the ? group of L.distichum were divided into4subgroups; the ? group of L.cernuum were divided into2subgroups. The analysis of the R clustering indicated that32characters in the experiment were independent of each other. And the number that the character of basic classification is reasonable.5. From the principal component analysis,32characters can be summarized in5principal components by principal component analysis, the total cumulative contributing rates of5principal components reached up to79.622%. The contribution ratio of principal component1was the first, the characteristics with larger characteristic values were leaf width, plant height, stem diameter, flower diameter, Style long17characters.the result indicating that the first principal component is a comprehensive index composed of several actual observed value traits of different organs about leaf blade,flower,flowers form factors; The second was principal component2, the characteristics with larger characteristic values were the leaves aspect ratio, leaf form, fragrance, the color of the flowers7characters of the leaf and flower, they are also the important characteristic values; Among the third principal component, anther long,flower spotted size, the outer petals aspect ratio are important characteristic values, it is mainly characteristic traits of the flower petal organ; Among the fourth principal component, there are the flowers, leaf length, stigma long and the color of the sterm characters; Among the fifth principal component, the Spotted number of the flower is the important characteristic value.Analysis the result we can see that select other4principal components appropriately based on the selection of the principal component1, you can achieve the desired results.6.The genetic relationship and change in diversity of the4species of Lilium. the results of ISSR markers and quantitative classification clustering analysis showed that:Between the ISSR marker results and the quantity classification results of the Lily materials we used, there has a certain similarity, among the4Lily the L.leicbtlinii var.maximowiczii have closest relationships with the L.dauricum from the Zhong Hua group,and closer with the L.distichum from the Round leaf Group, but they are far away from the L.cernuum in the same Volumeflap group. The two method showed that the samples who were collected from the same region or collected from the provenance neighborhood have the close genetic relationship. |
PDF Full Text Request