Changbai Mountain Reserve Amphibian Iridovirus Space Distribution And Ecological Security Research

Amphibians in the context of the global decline continues, some population even moved towards extinction. Amphibians is an important part of the food chain. Amphibians population decline and many factors, such as global warming, habitat fragmentation, ultraviolet light and some human interference, but the spread of the disease is not allow to ignore. A kind of amphibians are spread widely, and high death rate of virus disease-rainbow virus disease has become the influence of amphibians population is an important factor.Changbai mountain nature reserve is of the two countries and JieShan, is the tumen river, the yalu river, the songhua river three sources. Frog virus except for the between individual travel directly to the outside, the main transmission way is water and sediment in the water. Investigation on the white clear protection area in the rainbow virus infection can not only make clear changbai mountain game reserve the survival condition of amphibians, also know to sanjiang tributaries flows through the rainbow virus to provide the basis.In this study, we samplingin241samples in changbai mountain nature reserve including117Rana dybowskii,69Bufo bufogargarizans and55Salamandrella keyserlingii.. And in changbai mountain-odd arteries--the liaodong peninsula area acquisition65amphibians, including37Rana nigromaculatta Hallowellonly,the23Rana dybowskii, the5Bufo bufogargarizans. In changbai mountain nature reserve covers a total of11place, Contains almost all of the changbai mountain area with vertical distribution. And simple analysis the amphibians in vertical distribution of species composition with and the different growth situation. This study GuoHongCai virus specificity amplification zhongtong primer for all test samples for the tests,it showed that only at an altitude of1281m premises to the northeast of collection of detection to two strains had positive results, In changbai mountain-odd arteries--the liaodong peninsula area did not test positive results.We told the two strains of the virus which northeast source frog MCP gene sequences for further analysis, and with the known in Europe, America, Australia and other countries of the sequences comparison, carries on the system development analysis, in system development analysis. The results found that these two strains of virus and the frog in Europe and Australia sequence of blood is far away, and frogs of representative of the virus strain FV3related to sex is nearly, so identified as frogs FV3virus.For clinical diagnosis of time found a northeast brown at the right and the abdomen samples in the dark grey texture jaw a hard warts bag,.Back to the laboratory it wax block of embedding biopsy, but did not find a rainbow of the existence of virus, just general suppurative inflammation. Will changbai mountain reserves in water samples to identify out of the algae to carry on the analysis. Found in changbai mountain reserve water quality, in addition to frequent human activities outside the district, other parts of the water quality are water purification, did not find instructions pollution species. To protect the natural environment that is still very raw.This research system and full of changbai mountain reserve of amphibians the rainbow virus investigation, Results only found two strains of positive samples. In the kinds of algae in the analysis also did not find instructions pollution. This suggests that changbai mountain nature reserve of the protection of the environment to be good. This is not only to protect the work before we certainly, but also to the protection of our future work put forward higher request.