Effect Of Enterococcus Faecalis On Growth Performance, Meat Quality And Plasma Metabolism In Broiler Chickens

The study was used AA broiler chickens as experiment animals, for researching effect on growth performance, slaughter performance, meat quality, antioxidant capacity, nutrients utilization and plasma metabolism after supplementation of Enterococcus faecalis in broiler chickens feed. Results provided the theory basis for practical application of Enterococcus faecalis in poultry production.Test one: Effect of Enterococcus faecalis on production performance, meat quality, and antioxidant capacity in broiler chickensThis experiment was conducted to research the effect of Enterococcus faecalis on growth performance, slaughter performance, meat quality, antioxidant parameters, and nutrients utilization from AA(Arbor Acres) broiler chickens. There were 180 AA broiler chickens(coc, 1day) were randomly assigned to 3 groups according to weight equilibrium principle. Each group had 6 replicates which there were 10 chickens in each replicate. Group I(control group) was fed a basal diet, and group II and III were fed the basal diet added with 1×108 CFU/kg and 5×108 CFU/kg Enterococcus faecalis(viable count was 1×1010CFU/g), respectively. The experiment was lasted for 42 days.The results showed as follows: compared with the group I, 1) Added 5×108CFU/kg Enterococcus faecalis to the diet significantly decreased the ratio of feed to gain in the later period(22-42 day)(P<0.05) and the percentage of abdominal fat in the whole period(P<0.05), and significantly increased the percentage of half-eviscerated yield in the whole period(P<0.05); 2) Added 5×108CFU/kg Enterococcus faecalis to the diet significantly improved muscle fat and inosine monophosphate(IMF) content of breast muscle and leg muscle at 42 days, increased monounsaturated fatty acids and polyunsaturated fatty acids content of breast muscle(P<0.05), increased unsaturated fatty acids and monounsaturated fatty acids content of leg muscle(P<0.05), significantly reduced saturated fatty acids content of leg muscle(P<0.05); 3) Added 5×108CFU/kg Enterococcus faecalis to the diet significantly increased total antioxidant capacity(T-AOC) of plasm, liver, breast muscle and leg muscle, significantly increased total superoxide dismutase(T-SOD) and glutathione peroxidase(GSH-Px) capacity of plasm and liver, and significantly decreased lipid peroxide(LPO) and malondialdehyde(MDA) content of plasm, liver, breast muscle and leg muscle at age of 42 days(P<0.05); 4)Added 5×108CFU/kg Enterococcus faecalis to the diet significantly increased broiler chickens energy and protein utilization rate in the whole period. Conclusion: supplementation of Enterococcus faecalis in the broiler diet had an beneficial effect on production performance, meat quality and antioxidant capacity of 1-42 d broiler chickens Test two: Effect of Enterococcus faecalis on plasm metabolism in broiler chickensThis study used metabonomics analysis techniques, which connected liquid chromatography, gas chromatography with mass spectrometry(LC-MS,GC-MS), to research the effect of different doses Enterococcus faecalis in the diet on plasm metabolism in broiler chickens. After processed the plasma sample of AA broiler chickens, we detected its plasma metabolites, and identified plasma metabolites by mass spectrometry database.The results showed as follows: 1)compared with the group I, added 5×108 CFU/kg Enterococcus faecalis significantly changed the relative content of plasma metabolites(P < 0.05), mainly were Phosphatidyl Cholines(14:0), Hemolysis Phosphatidyl Ethanolamine(22:5), PC(36:6)/PE(39:6), Phosphatidy Inositol(18:0), Phosphatidic acid(22:6), PC(26:1)/PE(29:1), Phosphatidylglycerol(18:2), Hexadecanoic acid(16:0), Glycine, Fumaric acid, L-Threonine, Malic acid, D-Glucose, D-Galactose, and Monostearin; added 1×108 CFU/kg Enterococcus faecalis also significantly changed the relative content of plasma metabolites(P < 0.05), but its mainly were Phosphatidyl Ethanolamine(20:5), 1-Linoleoyl glycerol phosphocholine, Hemolysis Phosphatidyl Ethanolamine(22:5), Phosphatidyl Ethanolamine(20:3), Phosphatidyl Cholines(16:0), Phosphatidyl Cholines(20:3), PC(36:6)/PE(39:6), Phosphatidyl Cholines(40:7), Phosphatidyl Ethanolamine(18:3), Glycerophospho-N-Arachidonoyl Ethanolamine, PC(26:1)/PE(29:1), Phosphoric acid, Phosphate, Rythronic acid, D-Glucose, Palmitelaidic acid, à-D-Glucopyranoside, Hexadecanoic acid, Cholesterol, D-Fructose, d-Glucose/d-Galactose, 9-Octadecenoic acid, Melibiose;2)Compared 5×108 CFU/kg Enterococcus faecalis with 1×108 CFU/kg Enterococcus faecalis the relative content of plasma metabolites were significantly changed(P<0.05)mainly are Phosphatidyl Cholines(20:5), PC(18:0)/PE(21:0), Hemolysis Phosphatidyl Ethanolamine(22:5), 1-Linoleoyl glycerol phosphocholine, Phosphatidyl Cholines(20:5), Phosphatidyl Cholines(20:3), PC(17:2)/ PE(20:2), Glycine, Fumaric acid, Malic acid, D-Fructose and Galactopyranose. Conclusion: after supplementation of Enterococcus faecalis in the broiler diet, it also had an positive effect of on broiler plasm metabolome, 35 species of potential metabolites existed statistical differences, among these substances, which related to meat quality, were Phosphatidyl Choline(PC), Phosphatidyl Ethanolamine(PE), Hemolysis Phosphatidyl Ethanolamine(Lyso PE), Phosphoric Acid, Hydroxyl Succinic Acid,D-glucose and Palmitic Acid.