Establishment Of Nitroufurans Detection In Feeds By High Performance Liquid Chromatography

Nitrofurans are important synthetic broad-spectrum antibacterial drugs, with the 5-nitryl-furan basic structure, such as Furazolidone, Furaltadone, Nitrofurantoin and Furacillin. Nitrofurans are widely used as the broad-spectrum antibiotics in feed additives and therapeutic drugs for preventing and treating the gastrointestinal disease caused by Salmonella and E. coli in pigs, cattle, poultry and bees. And they have demonstrated good prevention and treatment effectivenss in veterinary clinic. Because of the potential carcinogenic and mutagenic effects caused by nitrofurans and their metabolites, nitrofurans are prohibited using in all animal-producing breeding in China and all over the world. However, because of their high antibacterial efficiency, lower cost and other characteristics, nitrofurans still have being illegally used in animal husbandry and aquaculture.The aim of this study is to establish a high performance liquid chromatography (HPLC) method for determination of nitrofurans in feeds, according to the equipment levels of feed quality inspection agencies in China. Extraction used acetonitrle and cleaned up with HLB solid-phase extraction column, Nitrofurans were separated on Water Sunfire C18 column with UV detector 365 run, using acetonitrile and ammonium acetate buffer as mobile phase. The linearity range is good. The nitrofurans standard curve regression coefficient is above 0.9997, the linearity ranged from 0.2?30 ?g/mL. The average recoveries of nitrofurans ranged from 70%-98%, with RSD below 10%. This method exhibits easy operation, good linearity, high sensitivity and good reproducibility for determination of nitrofurans residues in formula feeds, concentrated feeds and premixes feed.