Effects Of Simulated Typhoon Conditions On The Expression Of Pinctada Martensii 5 Functional Genes

Hainan is an important pearl oyster culture zone in China, in which the breeding of pinctada martensii is the most widespread. Typhoon is a common natural disaster in Hainan area. After typhoon, the growth of pinctada martensii will be arrested for a period of time. At present, no studies have been reported on the relationship between typhoon attacks in tropical oceans, relevant functional gene expressions in pinctada martensii and "arrest" of growth. It is necessary to carry out such studies and clarify the causes of "arrest" of growth in pinctada martensii at a molecular level. In this paper, two main factors affecting the growth of pinctada martensii under typhoon conditions were simulated in the laboratory:water flow impact and sediment suspension. Pinctada martensii was treated and a control group was established. The expression levels of two oxidative stress genes, Prx and GPX, myostatin gene (MSTN), as well as two mineralization genes, N16 and prismalin-14 in corresponding pinctada martensii tissues were sampled and tested at 2 h,4 h,6 h,8 h,12 h and 24 h. The research results are as follows:1. Under water flow impact treatment, both the expressions of Prx and GPX in pinctada martensii gill tend to rise and then fall. At 4h, the expression levels of two genes are significantly higher than those of the control group. Under sediment suspension treatment, the expression of Prx is significantly higher than that of the control group at 2h, suggesting that both treatments will induce overexpression of two oxidative stress genes in pinctada martensii gill, in response to the adverse environment.2. Under water flow impact treatment, the expression of MSTN in pinctada martensii adductor muscle is significantly higher than that of the control group at 4 h. While at 4 h,6 h and 8 h, the expression of MSTN gene in pinctada martensii gill is significantly higher than that of the control group. Water flow impact may stimulate the expression of MSTN in pinctada martensii adductor muscle to go up and inhibit the growth of muscle cells, so as to adapt to environmental changes. Such a reaction is more obvious in gill tissues than adductor muscle. Under sediment suspension treatment, the expression of MSTN in pinctada martensii adductor muscle doesn't differ significantly from that of the control group. While the expression of MSTN in pinctada martensii gill at 2 h,4 h and 8 h was significantly higher than that of the control group, suggesting that sediment suspension treatment has a positive induction effect on the expression of MSTN in pinctada martensii gill, but has no effect in pinctada martensii adductor muscle.3. Under water flow impact treatment and sediment suspension treatment, the expression level of prismalin-14 gene in pinctada martensii mantle doesn't differ significantly from that of the control group. Under water flow impact treatment and sediment suspension treatment, the expression level of N16 gene in pinctada martensii mantle doesn't differ significantly from that of control group, suggesting that two treatments have no significant impact on the expression of two mineralization genes in pinctada martensii mantle.