Construction Of A Liquid Microbial Agents And Its Fertilizer Efficiency Research

A type of new efficient liquid mixed microbial agents with seven strains was developed in this experiment,which based on four parts: antagonism among strains,medium all strains growing,construction of mixed microbial agents and its Fertilizer Efficiency Research.The results showed that:(1)Antagonism between two kinds of strains is measured with agar disk.The results showed that Streptomyces microflavus had antagonism to five kinds of strains,in addition,only the antagonism of B.licheniformis on Bacillus subtilis,Paenibacillus kribbensis and Saccharomyces cerevisiae reached the C value of 1.20.(2)The influence of glucose concentration on OD value had been researched in beef extract peptone medium added wheat malt powder.The results showed that within certain realms,with the increasing of glucose concentration B.licheniformis OD value increased,the rest of the five kinds of strains OD value were 6.40g/L>9.60g/L>3.20g/L.Meanwhile,the influence of pH on strains OD value was researched,which showed that the OD values of Paenibacillus kribbensis,Saccharomyces cerevisiae and Azotobacter chroococcum were larger in pH 7.0.The OD value of Bacillus subtilis and B.licheniformis were larger in pH 7.5,the difference between7.5 and 7.0 can’t be told obviously.To estimate the effect of new medium deeply,the OD values of the strains in new and old medium were compared,which showed that the OD values of Bacillus subtilis,B.licheniformis,Paenibacillus kribbensis and Azotobacter chroococcum in new medium were all greater than old medium at 10 h,20h,30 h.The OD values of Saccharomyces cerevisiae in new and old medium at 30 h were nearly and had no insignificant difference;Lactobacillus acidophilus at 20 h is the same.(3)The liquid mixed microbes have good effect after 4 days,when adding Bacillus subtilis,B.licheniformis,Paenibacillus kribbensis,Lactobacillus acidophilus,Saccharomyces cerevisiae,Azotobacter chroococcum at the ratio of 2:1:2:2:2:2 in the new and 37℃ medium.(4)The effects of the liquid compound microbial agents(they was diluted 10 times,100 times and 1000 times)on wheat seed germination and seedling growth were researched under hydroponic condition.The results showed: In terms of germination,1000 times had the best effect,the germination percentage,germination energy and germination index were increased by 8.30%,8.30% and 5.94%,but 10 times inhibited germination obviously.In terms of seedling growth,1000 times promoted plant height,the length of both sides root and root activity obviously,they were increased by 9.78%、21.59% and 56.60%.The effects of the three dilution ratio liquid compound microbial agents and its fermentation solution on lettuce seed germination and plant height were researched under hydroponic condition.The results showed: In terms of germination,1000 times fermentation solution improved germination percentage significantly by 32.2%,this was about double of 1000 times microbial agents.10 times microbial agents reduced germination percentage significantly by 25%.This showed that the key factor to promote germination was fermentation solution,high concentration surviving bacteria inhibited lettuce seed germination.In terms of seedling growth,the dilution ratio of 1000 microbial agents and its fermentation solution were both best,increased respectively 42.86% and 27.62%,which had no significant difference with the control.The seedling rate,plant height and the longest leaf of corn increased by 21.70%,21.97% and 17.58% respectively in the soil added microbial agents.According to above all,the liquid mixed microbes have good effect after 4 days,when adding Bacillus subtilis: B.licheniformis: Bacillus mucilagenosus: Lactobacillus acidophilus: Saccharomyces cerevisiae: Azotobacter chroococcum at the ratio of 2:1:2:2:2:2 in the new and 37℃ medium which includes 3g beef extract,10 g peptone,5g Na Cl,6.4g glucose,10 g wheat malt powder,distilled water 1000 mL,and pH 7.0.