Association Analysis For Wheat Potassium Efficiency Traits Based On Molecular Marker Of SNP

Potassium is one of the indispensable nutrient element. Cultivating the potassium efficient wheat varieties, making full use of potassium in the soil is a basic way to alleviate the shortage of potash resource in china. In this study, varieties of wheat were used as the material. Seedling experiments were finished by hydroponic culture, havest stage experiments were carried out in the field. All the population were grown in low potassium?LK? conditions as a treatment and in normal potassium?MK? conditions as a control. Effects of potassium deficiency on K utilization efficiency at seedling and mature period and final yield were studied. The association analysis of wheat K efficiency-related traits and yield-related traits with a mass of molecular markers of SNP were carried on, and some important sites of K nutrition-related traits were detected and which offer some reference for wheat breeding. The main results were as follows:?1? Under the two LK treatments, the plant shoot dry weight, the plant total dry weight and the straw weight per plant at the seedling stage were all significantly decreased. However, the root shoot ratio were higher under low level of potassium, Under low potassium treatment in the environment, the cumulative amount of potassium was relatively low while the K utilization efficiency showed an increase tendency.?2? Association analysis showed that 1,102 sites were detected at seedling stage, including 137 stable association sites related with at least two traits. Single markerer loci phenotypic variation ranged from 7.5% to 22.63%. Four markers were screened to significantly associated with six or seven traits. These Four markers were wsnp_K u_c13311₂1255428, Excalibur_c8670₉72, IACX5989 and wsnp_Ex_c12887₂0426781. These markers were located on chromosome 3B, 6D, 4B, 3B respectively.?3? 1,874 sites were detected at mature period, including 223 stable association sites were associated with two or more traits. Single markerer loci phenotypic variation ranged from 7.43% to 19.76%. Five markers were screened to significantly associated with six or more traits. These Five markers were Excalibur_rep_c111263₃07?6A?, Bob White_c23839₁19?6A?, Excalibur_c115824₂67?6A?, Excalibur_c2287₁327?6A?, IAAV2681?6A?.?4? There were nine stable markers detected in both seedling and mature periods. RFL_Contig2834₈90?7A? could be detected only in normal conditions. RAC875_c7123₁703 could be detected only in low K conditions. They were located on chromosome 7B. Tdurum_contig54649₇98?2B?, IAAV2823?4A?, CAP8_c1881₂15?6A?, Ex_c13223₁847?6A?, RFL_Contig5170₃30?6A?, tplb0060j17₄50?6A? and wsnp CAP11_c1594₈82280?6A?, could be detected under normal or low phosphorus conditions.